Human CellExp™ GFER/HPO/ALR, human recombinant

• Catalog number
  7269-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  GFER, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS, Hepatopoietin
• Alternative_names
  GFER, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS, Hepatopoietin
• Description
  Acts as an autocrine hepatotrophic growth factor
• Recombinant
  Yes
• Source
  HEK 293 cells
• Purity by SDS PAGE
  ≥92%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Molecular Weight
  Calculated MW of 15.2 kDa with no tag. The predicted N-terminus is Met 81. DTT-reduced protein migrates as 16.0 kDa.
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Lyophilized from 0.22 um filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
• Background Information
  Growth factor, augmenter of liver regeneration (GFER) is also known as FAD-linked sulfhydryl oxidase ALR, which belongs to the Erv1/ALR family of proteins. This family can be found in higher and lower eukaryotes. There are two isoform of GFER: Isoform 1 and isoform 2. Isoform 2 missing 1 – 80 aa. Isoform 1 is mainly located in mitochondrion intermembrane space, while Isoform 2 is secreted to cytoplasm. Isoform 1 of GFER regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. The isoform 2 of GFER may act as an autocrine hepatotrophic growth factor promoting liver regeneration.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  CellExp™   GFER/HPO/ALR  
• Gene symbol
  GFER, AKR1A1, KMT2D
• Short name
  CellExp™ GFER/HPO/ALR, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Humans
• Alternative name
  H. sapiens CellExp™ GFER/HPO/ALR, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4236
• Gene
  GFER
• Long gene name
  growth factor, augmenter of liver regeneration
• Synonyms gene name
  • growth factor, erv1 (S. cerevisiae)-like (augmenter of liver regeneration)
• Synonyms
  • HSS
  • ERV1
  • ALR
  • HERV1
  • HPO1
  • HPO2
• Synonyms name
  • ERV1 homolog (S. cerevisiae)
  • FAD-linked sulfhydryl oxidase ALR
  • hepatic regenerative stimulation substance
• GenBank acession
  • BC002429
• Locus
  16p13.3
• Discovery year
  1997-03-19
• Entrez gene record
  2671
• Pubmed identfication
  • 8575761
• RefSeq identity
  • NM_005262
• VEGA ID
  OTTHUMG00000176896

Gene info

• Identity
  HGNC:380
• Gene
  AKR1A1
• Long gene name
  aldo-keto reductase family 1 member A1
• Synonyms gene name
  • aldo-keto reductase family 1, member A1 (aldehyde reductase)
• Synonyms
  • ALR
  • DD3
• Synonyms name
  • dihydrodiol dehydrogenase 3
  • aldehyde reductase
• GenBank acession
  • J04794
• Locus
  1p34.1
• Discovery year
  1998-09-29
• Entrez gene record
  10327
• Pubmed identfication
  • 2498333
  • 10393438
• RefSeq identity
  • NM_006066
• Classification
  • Aldo-keto reductases
• VEGA ID
  OTTHUMG00000007740

Gene info

• Identity
  HGNC:7133
• Gene
  KMT2D
• Long gene name
  lysine methyltransferase 2D
• Synonyms gene
  • TNRC21
  • MLL2
• Synonyms gene name
  • trinucleotide repeat containing 21
  • myeloid/lymphoid or mixed-lineage leukemia 2
  • lysine (K)-specific methyltransferase 2D
• Synonyms
  • ALR
  • MLL4
  • CAGL114
• Synonyms name
  • histone-lysine N-methyltransferase 2D
• GenBank acession
  • AF010403
• Locus
  12q13.12
• Discovery year
  1998-10-14
• Entrez gene record
  8085
• Pubmed identfication
  • 9247308
• Classification
  • PHD finger proteins
  • SET domain containing
  • Lysine methyltransferases
• VEGA ID
  OTTHUMG00000166524

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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