Human CellExp™ GFER/HPO/ALR, human recombinant
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Catalog number7269-10
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PricePlease ask
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Size10 ug
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SynonymsGFER, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS, Hepatopoietin
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Alternative_namesGFER, ALR, ERV1, HERV1, HPO, HPO1, HPO2, HSS, Hepatopoietin
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DescriptionActs as an autocrine hepatotrophic growth factor
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RecombinantYes
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SourceHEK 293 cells
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Purity by SDS PAGE≥92%
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AssaySDS-PAGE
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Endotoxin Level< 0.1 ng/ug of protein (
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Molecular WeightCalculated MW of 15.2 kDa with no tag. The predicted N-terminus is Met 81. DTT-reduced protein migrates as 16.0 kDa.
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionLyophilized from 0.22 um filtered solution in 20 mM Tris, 100 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 100 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
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Background InformationGrowth factor, augmenter of liver regeneration (GFER) is also known as FAD-linked sulfhydryl oxidase ALR, which belongs to the Erv1/ALR family of proteins. This family can be found in higher and lower eukaryotes. There are two isoform of GFER: Isoform 1 and isoform 2. Isoform 2 missing 1 – 80 aa. Isoform 1 is mainly located in mitochondrion intermembrane space, while Isoform 2 is secreted to cytoplasm. Isoform 1 of GFER regenerates the redox-active disulfide bonds in CHCHD4/MIA40, a chaperone essential for disulfide bond formation and protein folding in the mitochondrial intermembrane space. The reduced form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with GFER/ERV1, resulting in regeneration of the essential disulfide bonds in CHCHD4/MIA40, while GFER/ERV1 becomes re-oxidized by donating electrons to cytochrome c or molecular oxygen. The isoform 2 of GFER may act as an autocrine hepatotrophic growth factor promoting liver regeneration.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolGFER, AKR1A1, KMT2D
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Short nameCellExp™ GFER/HPO/ALR, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHumans
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Alternative nameH. sapiens CellExp™ GFER/HPO/ALR, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene namegrowth factor, augmenter of liver regeneration
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Synonyms gene name
- growth factor, erv1 (S. cerevisiae)-like (augmenter of liver regeneration)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-03-19
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namealdo-keto reductase family 1 member A1
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Synonyms gene name
- aldo-keto reductase family 1, member A1 (aldehyde reductase)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-09-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Aldo-keto reductases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namelysine methyltransferase 2D
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Synonyms gene
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Synonyms gene name
- trinucleotide repeat containing 21
- myeloid/lymphoid or mixed-lineage leukemia 2
- lysine (K)-specific methyltransferase 2D
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1998-10-14
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Entrez gene record
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Pubmed identfication
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Classification
- PHD finger proteins
- SET domain containing
- Lysine methyltransferases
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VEGA ID
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data