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Immunogen
A phospho-specific peptide corresponding to residues surrounding Ser174 of human KHS1 (AA171-177)
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Antibody s target
Human MAP4K5 (pSer174)
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Antibody s full description
Rabbit Anti-Human MAP4K5 (pSer174) Polyclonal
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Antibody s category
Polyclonal Antibodies
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Antibody s other name
KHS Antibody, Kinase homologous to SPS1/STE20 Antibody, MAPK/ERK kinase kinase kinase 5 Antibody, MEK kinase kinase 5 Antibody, MEKKK 5 Antibody
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Verified applications
WB, AM
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Raised in
Rabbit
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Antibody s reactivity
Human
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Antibody s recommended dilutions for use
WB (1:250); optimal dilutions for assays should be determined by the user.
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Antibody s purified from
Peptide Affinity Purified
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Recommended buffer for storage
PBS pH7.4, 50% glycerol, 0.025% Thimerosal
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Antibody s concentration
See included datasheet or contact our support service
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Antibody s specificity
Detects 95.04 kDa.
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Storage recommendations
-20°C
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Shipping recommendations
Blue Ice or 4°C
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Certificate of analysis
A 1:250 dilution of SPC-1002 was sufficient for detection of MAP4K5 (pSer174) in 10 µg of Jurkat cell lysate by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
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Antibody in cell
Cytoplasm
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Tissue specificity
Ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate
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Scientific context
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
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Bibliography
Refer to PubMed
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Released date
29/Aug/2016
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NCBI number
NP_006566
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Gene number
11183
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Protein number
Q9Y4K4
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PubMed number
Refer to PubMed
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Tested applications
To be tested
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Tested reactivity
To be tested
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Antibody s datasheet
Contact our support service to receive datasheet or other technical documentation.
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Representative figure link
No Data Available
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Warning information
Non-hazardous
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Country of production
Canada
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Total weight kg
1.4
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Net weight g
0.1
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Stock availabilit
In Stock
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Test
StressMark antibodies supplies antibodies that are for research of human proteins.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera. Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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Gene target
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Gene symbol
MAP4K5, APC
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Short name
Rabbit Anti- MAP4K5 (pSer174) Polyclonal: APC
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Technique
anti-Human, Polyclonal, Rabbit, anti-, anti, antibody to, antibodies, antibodies against human proteins, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Host
Rabbit, Rabbits
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Isotype
Immunoglobulin
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Label
APC
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Species
Human, Humans
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Alternative name
production species: rabbit Antibody toHuman mitogen-activated protein kinase kinase kinase kinase 5 (pSer174) polyclonal: adenomatous polyposis coli
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Alternative technique
polyclonals, rabbit-anti, antibodies
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Alternative to gene target
mitogen-activated protein kinase kinase kinase kinase 5, GCKR and KHS and KHS1 and MAPKKKK5, MAP4K5 and IDBG-6029 and ENSG00000012983 and 11183, transferase activity, Plasma membranes, Map4k5 and IDBG-144865 and ENSMUSG00000034761 and 399510, MAP4K5 and IDBG-646448 and ENSBTAG00000014792 and 781335
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