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Long name
Rabbit Antibody against Chlamydia trachomatis omcB, Biotin
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Synonym name
omcB Chlamydia trachomatis Antibody
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Species reactivity
Chlamydia trachomatis
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Tested applications recommended dilutions
ELISA
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Conjugation
Biotin
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Antibody isotype
IgG
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UniProt ID
P0CC04
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Format
Liquid, diluted in buffer
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Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
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Purity
>95%
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Purification method
Protein G purified
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Antibody category
Polyclonal Antibodies
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Immunogen type
Recombinant Chlamydia trachomatis Large cysteine-rich periplasmic protein OmcB protein (15-243AA)
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Immunogen species
Chlamydia trachomatis
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Alias
Large cysteine-rich periplasmic protein OmcB,Large-CRP,60 kDa cysteine-rich OMP,60 kDa outer membrane protein,Cysteine-rich outer membrane protein,CRP,omcB,omp2,omp2B,CT_443
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Background
In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the major outer membrane protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan (By similarity).
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Clonality
Polyclonal antibody
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Shipping and storage
omcB Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
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Short name of the antigen
Large cysteine-rich periplasmic protein OmcB
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Additional conjugation
Biotinylated
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About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
