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Synonyms
Caldecrin, Chymotrypsin C, Chymotrypsin-C, Chymotrypsin C, CLCR, CTRC, CTRC_HUMAN, ELA 4, ELA4, Elastase 4, Elastase IV, Elastase4, Elastase IV, Serum calcium decreasing factor
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Alternative_names
Caldecrin, Chymotrypsin C, Chymotrypsin-C, Chymotrypsin C, CLCR, CTRC, CTRC_HUMAN, ELA 4, ELA4, Elastase 4, Elastase IV, Elastase4, Elastase IV, Serum calcium decreasing factor
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Description
A serine peptidase that selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine.
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Recombinant
No
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Source
Human Pancreas
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
40-70 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of Suc-Ala-Ala-Pro-Phe-pNA/min at 21°C, pH 8.0.
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Results
40-70 units per mg protein.
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Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes one µmole of Suc-Ala-Ala-Pro-Phe-pNA/min at 21°C, pH 8.0.
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Molecular Weight
25.0 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized as a salt-free solid.
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Background Information
Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
