APRIL, Mouse Recombinant

• Catalog number
  7118-10
• Price
  Please ask
• Size
  10 ug

• Synonyms
  A Proliferating-inducing Ligand, TNFSF13, TRDL-1α
• Alternative_names
  A Proliferating-inducing Ligand, TNFSF13, TRDL-1α
• Description
  Stimulates proliferation of various tumor cell lines like Jurkat T cells and MCF-7 carcinoma cells.
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 0.1 ng/ug of protein (
• Activity Specifications test method
  Determined by its ability to induce cell proliferation of activated T-cells.
• Biological activity
  Determined by its ability to induce cell proliferation of activated T-cells.
• Molecular Weight
  21.9 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Lyophilized powder
• Physical form description
  Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Phosphate, pH 5 and 100 mM Arginine.
• Reconstitution Instructions
  Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
• Background Information
  APRIL, a member of the TNF superfamily, is expressed in monocytes, macrophages, certain transformed cell lines, certain cancers of colon, and lymphoid tissues. APRIL, along with another TNF family member, BAFF, compete for two receptors, TACI and BCMA. ARPIL has the ability to stimulate proliferation of various tumor cell lines including Jurkat T cells and MCF-7 carcinoma cells. Like BAFF, APRIL also stimulates the proliferation of B and T cells. The human APRIL gene codes for at least four alternatively spliced transcriptional variants, which give rise to different isoforms of the APRIL precursor protein. All isoforms can be cleaved by the protease, furin, to release a soluble C-terminal fragment, which comprises the TNF like receptor binding of the APRIL precursor. Recombinant murine APRIL is a soluble 21.9 kDa protein, consisting of 192 amino acid residues.
• Amino acid sequence
  MRREVSRLQR SGGPSQKQGE RPWQSLWEQS PDVLEAWKDG AKSRRRRAVL TQKHKKKHSV LHLVPVNITS KDSDVTEVMW QPVLRRGRGL EAQGDIVRVW DTGIYLLYSQ VLFHDVTFTM GQVVSREGQG RRETLFRCIR SMPSDPDRAY NSCYSAGVFH LHQGDIITVK IPRANAKLSL SPHGTFLGFV KL
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  APRIL  
• Gene symbol
  TNFSF13, ANP32B
• Short name
  APRIL, Mouse Recombinant
• Technique
  Recombinant, Mouse, mouses, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  mouse
• Species
  Mouse, Mouses
• Alternative name
  APRIL, Mouse Rec.
• Alternative technique
  rec, murine

Gene info

• Identity
  HGNC:11928
• Gene
  TNFSF13
• Long gene name
  TNF superfamily member 13
• Synonyms gene name
  • tumor necrosis factor (ligand) superfamily, member 13
• Synonyms
  • APRIL
  • CD256
• GenBank acession
  • AF046888
• Locus
  17p13.1
• Discovery year
  1998-12-04
• Entrez gene record
  8741
• Pubmed identfication
  • 9743536
• RefSeq identity
  • NM_003808
• Classification
  • Tumor necrosis factor superfamily
  • CD molecules
• VEGA ID
  OTTHUMG00000108145

Gene info

• Identity
  HGNC:16677
• Gene
  ANP32B
• Long gene name
  acidic nuclear phosphoprotein 32 family member B
• Synonyms gene name
  • acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
• Synonyms
  • SSP29
  • PHAPI2
  • APRIL
• Synonyms name
  • acidic protein rich in leucines
• GenBank acession
  • Y07969
• Locus
  9q22.33
• Discovery year
  2002-02-13
• Entrez gene record
  10541
• Pubmed identfication
  • 9285060
  • 9473664
• RefSeq identity
  • NM_006401
• Classification
  • ANP32 acidic nuclear phosphoproteins
• VEGA ID
  OTTHUMG00000020338

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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