FITC-Linked Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1)
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Catalog number
MBS2042959
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Price
Please ask
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Size
0,1 mg
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Other size
please contact us to order other different size
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Properties
If you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Description
Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
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Conjugation
Anti-FITC Antibody
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Group
Polyclonals and antibodies
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About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
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French translation
anticorps
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Gene target
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Gene symbol
ECE1, ECE1-AS1
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Short name
FITC-Linked Polyclonal Antibody Endothelin Converting Enzyme 1 (ECE1)
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Technique
Polyclonal, Antibody, Enzyme, FITC, antibodies against human proteins, antibodies for, enzymes, Fluorescein, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
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Label
FITC
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Alternative name
fluorecein-Linked polyclonal (antibody to-) to Endothelin Converting Enzyme 1 (endothelin converting enzyme 1)
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Alternative technique
polyclonals, antibodies, enzymes, fluorescine
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Alternative to gene target
endothelin converting enzyme 1, ECE, ECE1 and IDBG-93094 and ENSG00000117298 and 1889, metal ion binding, Cell surfaces, Ece1 and IDBG-199213 and ENSMUSG00000057530 and 230857, ECE1 and IDBG-645619 and ENSBTAG00000002977 and 281133
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Gene info
Gene info
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Identity
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Gene
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Long gene name
ECE1 antisense RNA 1
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Locus
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Discovery year
2019-07-25
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Entrez gene record
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RefSeq identity
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Classification
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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