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Available ordering format
Lyophilized in phosphate buffer saline
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Immunogen
phospholipase D isolated and purified from Brassica oleracea
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified IgG
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How to reconstitute
For reconstitution please add 0.5 ml of sterile destilled water.
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
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Connected products
AS09 556 | anti-PLD phospholipase DAS09 557 | anti-PLD phospholipase D (biotinylated)Plant protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 100 - 1: 1000 for techniques listed above
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Molecular weight expected аpparent
92 kDa
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Verified reactivity
Brassica oleracea
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Possible reactivity
dicots including: Arabidopsis thaliana, Cucumis melo, Ricinus communis, trees: Populus balsamifera
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
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References
to be added when available
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Scientific context
Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more. Alternative name: phosphatidylcholine phosphatido-hydrolase.
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Notes
antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Test
A high affinity purification column was use to purify PLD, phospholipase D, by agisera by chromatographic size exclusion.
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Description
1 of protein A or G purified by agisera will give more specificity than crude serum.
