JK1 Feeder Cells

We send this reagant on

Dry Ice
The temperatureerature for storage is

Liquid nitrogen
Technical file
SDS or safty data sheet

more (CBA-310, 311, 312, 313, 315, 316) GHS.pdf
Type of reagent

Stem Cell Research
Related techniques

Stem Cell Research, Feeder Cells, JK1 Passage-Independent Feeder Cells
Use of the reagent

JK1 Feeder Cells are used for the maintenance of numerous types of stem cells in their undifferentiated state. The cells must be mitotically inactivated prior to the addition of ES cells, such as treatment with mitomycin C (2-4 hr, 10 µg/mL).
Cellular use

Support longer-term proliferation compared to MEF feeder cells
Protocol information

Culture a wide variety of cell types including pluripotent ESCs, germ-line derived stem cells (e.g. SPCs and MASCs), and primordial germ cell-derived EGCs
Reagent properties

Must be mitotically inactivated prior to addition of stem cells
Conjugation and form

host specific
Species compatible to

human cells and cell lines and other cells
Use of this test kit or product

Molecular biology
Antigen

the sequence of the protein
Buffer

The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,67
Molecular weight

kiloDalton
This reagent is purified

0.98

Identity

HGNC:25964
Gene

RETREG1
Long gene name

reticulophagy regulator 1
Synonyms gene
- FAM134B
Synonyms gene name
- family with sequence similarity 134, member B
- family with sequence similarity 134 member B
Synonyms
- FLJ20152
- JK1
GenBank acession
- BC053326
Locus

5p15.1
Discovery year

2007-05-01
Entrez gene record

54463
Pubmed identfication
- 19838196
- 24327336
RefSeq identity
- NM_001034850
VEGA ID

OTTHUMG00000161788
Locus Specific Databases
- LRG_363