Strep-Tag II Antibody

• Catalog number
  3988-100
• Price
  Please ask
• Size
  100 µg

• Antibody Target
  Strep-Tag II
• Target Alternative Name
  Strep-Tag II, anti-Strep-Tag II, Strep-Tag II antibody,Strep-Tag IIantibody
• Antibody Type
  Polyclonal
• Species Reactivities
  All species
• Immunogen Sequence
  A synthetic Strep-Tag II peptide NWSHPQFEK conjμgated to KLH.
• Appearance
  Colorless liquid
• Form
  Liquid
• Concentration
  0.5 mg/ml
• Formulation
  0.5 mg/ml affinity purified rabbit anti-Strep-Tag II polyclonal antibody in PBS, pH 7.2, 30% glycerol, 0.5% BSA, 0.5 mM EDTA and 0.03% proclin
• Purification
  Affinity purified
• Application
  Western blot
• Positive Control
  Strep-Tag II protein ladder (Lane 1)
• Application And Usages
  The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.
• Description
  Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Purpose Only. Not to be used in human subjects.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Strep-Tag  
• Short name
  Strep-Tag II Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for, Tags
• Isotype
  Rabbit IgG
• Label
  tag
• Alternative name
  Strep-detection labelled II (antibody to-)
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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