CD3/CD8/CD45 Antibody (FITC, PE, PerCP)
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Catalog number
abx200625
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Price
Please ask
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Size
50 tests
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Category
Combination Antibodies
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Clonality
Monoclonal, Monoclonal, Monoclonal
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Raised in
Mouse, Mouse, Mouse
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Target
CD3/CD8/CD45 Antibody (FITC, PE
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Immunogen
see included datasheet or contact us
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Species reactivity
Human, Mouse, Rat (for other species please inquire)
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Purification
Affinity purified
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Applications
WB (for other applications please contact us)
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Stock availability
Shipped within 5-12 working days.
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Storage conditions
Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
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Footnote
This product is for research use only.
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Properties
If you buy Antibodies supplied by Abbexa they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Abbexa Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Abbexa FITC is highly sensitive to pH extremes.
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Description
Cytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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Conjugation
Anti-FITC Antibody
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Conjugate
Peridinin chlorophyll (PerCP)
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French translation
anticorps
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Gene target
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Gene symbol
CD8A, PTPRCAP, PTPRC
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Short name
CD3/CD8/CD45 Antibody (FITC, PE, PerCP)
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Technique
Antibody, Percp, FITC, antibodies against human proteins, antibodies for, Fluorescein, Peridinin chlorophyll protein (PerCP) has a 677 nanometer maximum emission, red, when excited at 488 nanometers and the CD3/CD8/CD45 Antibody (FITC, PE, PerCP) is detected on the FL3 detector of a FACSCalibur or FACScan. A PerCP dye is also available (PerCP-Cy5.5, also written Cy5.5-PerCP). PerCP is not suited for the high-powered lasing (>150mW) applications, such as on a MoFlo, due to it photobleaching (features/ qualities/ traits). PerCP conjugates can only be received/be gotten from the Becton Dickinson/Pharmingen group of companies or from Abbexa.
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Label
FITC, PE, PerCP
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Alternative name
CD3/CD8/CD45 (antibody to-) (fluorecein, peroxidase, PerCP)
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Alternative technique
antibodies, antibodies-percp, fluorescine
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Gene info
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Identity
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Gene
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Long gene name
CD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year
1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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