Methylene blue 0.300 gm 95% ethyl alcohol 30.000 ml Potassium hydroxide 0.010 gm Distilled water 100.000 ml
M’Fadyean Stain (Polychrome Methylene Blue) is a readymade stain reported to be used for capsule staining of Bacillus anthracis
Deep violet coloured solution
Clear without any particles.
Store below 30°C in tightly closed container and away from bright light.
Bacillus anthracis ATCC 14578 : Pink capsule seen surrounding the deep bluish bacilli. Bacillus cereus ATCC 10876 : Deep bluish bacilli seen.
Use before expiry date on label.
Capsule staining is carried out using M'Fadyean stain and staining characteristic is observed under microscope using oil immersion lens.
For microbiological / pathological laboratories, hospitals, research settings and educational institutes. These stains augment tissue morphology, demonstrate important cellular components, aid in the evaluation of diseases, and identifies pathogenic organisms.
Blue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water.