CD25, IL-2Ra, Clone: MRC OX-39, Mouse Monoclonal antibody-Rat, Biotin; flow/ELISA (can use w/YSRTMCA494G)

  • Catalog number
    YSRTMCA273B
  • Price
    Please ask
  • Size
    vial
  • Alternative name 1
    CD25, IL-2Ra, Clone: MRC OX-39, Mab anti-Rat, Biotin; flow/ELISA (can use w/YSRTMCA494G)
  • Alternative name 2
    CD25, IL-2Ra, Clone: MRC OX-39, Mab antibody-Rat, Biotin; flow/ELISA (can use w/YSRTMCA494G)
  • Alternative name 3
    CD25, IL-2Ra, Clone: MRC OX-39, Mab antibody-Rattus norvegicustus norvegicus, Biotin; flow/ELISA (can use w/YSRTMCA494G)
  • Alternative name 4
    anti-CD25 IL-2Ra Clone: MRC OX-39 can use w/YSRTMCA494G
  • Alternative name 5
    CD25 IL-2Ra Clone: MRC OX-39 can use w/YSRTMCA494G
  • Clonality
    Monoclonal Antibody
  • Description
    This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
  • Storage and handling
    Generally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
  • Advisory
    To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
  • Properties
    If you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
  • Conjugation
    Biotinylated
  • Test
    ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED, Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • About
    Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Latin name
    Mus musculus, Rattus norvegicus
  • French translation
    anticorps
  • Gene target
  • Gene symbol
    IL36G, IL25, IL22, IL18, IL37, CD200, IL6R, IL17C, IL17A, IL17D
  • Short name
    CD25, IL-2Ra, Clone: MRC OX-39, Mouse Monoclonal antibody- , ; /ELISA (can w/YSRTMCA494G)
  • Technique
    Mouse monoclonal, Antibody, ELISA, Mouse, Flow, antibodies against human proteins, antibodies for, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
  • Host
    mouse monoclonal, antigen assay ELISA kit 1 Kit (96 Wells), Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
  • Label
    Biotin
  • Species
    Rat, Mouses, Rats
  • Alternative name
    CD25, Interleukin-2Ra, clonality: MRC OX-39, Mouse monoclonal (antibody to-)-Rat, biotinilated; flow/Enzyme-linked immunosorbent assay (can use w/YSRTMCA494G)
  • Alternative technique
    antibodies, murine, elisas
Gene info
Gene info
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    CD200 molecule
  • Synonyms gene
  • Synonyms gene name
    • antigen identified by monoclonal antibody MRC OX-2
    • CD200 antigen
  • Synonyms
  • Locus
  • Discovery year
    2001-06-22
  • Entrez gene record
  • Classification
    • CD molecules
    • Immunoglobulin like domain containing
  • VEGA ID
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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