Anti-Beta-2 Microglobulin Antibody (B2M/961)
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Catalog number
A1440-100
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Price
Please ask
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Size
100 µg
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Antibody Target
Beta-2 Microglobulin
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Target Alternative Name
B2M, Beta 2 microglobin, Beta 2 microglobulin, Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, Hdcma22p
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Antibody Type
Monoclonal
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Clone
B2M/961
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Species Reactivities
Human
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Immunogen Sequence
Full length recombinant human B2M protein
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Accession
P61769
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Gene Id
567
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Appearance
Colorless liquid
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Form
Liquid
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Formulation
In 10 mM PBS with 0.05% BSA & 0.05% azide
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Purification
Protein A/G purification
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Application
FC, IF, IHC
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Positive Control
IHC: Human cervical carcinoma, Human melanoma, Human endometrial carcinoma, Human bladder carcinoma, Human colon carcinoma, Human renal carcinoma
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Application And Usages
FC: 0.5-1 ug/1X106 cells, IF: 0.5-1 ug/ml, IHC: 0.5-1 ug/ml
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Description
Recognizes a protein of 12 kDa, identified as β-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an α heavy chain that contains three subdomains (α1, α2, α3) and a non-covalent associating light chain, known as β-2-Microglobulin. β-2-Microglobulin associates with the α3 subdomain of the α heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The α1 and α2 domains of the α heavy chain form the peptide antigen-binding cleft. Mutations in the β-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
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Storage Condition
-20°C
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Shipping Condition
gel pack
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Shelf Life
12 months
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Usage
For Research Use Only! Not to be used in humans.
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Handling
The antibody solution should be gently mixed before use.
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Western Blot Verified
Not Verified
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Immunocytochemistry Verified
Verified
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Immunofluorescence Verified
Not Verified
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Immunoprecipitation Verified
Not Verified
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FACS Verified
Not Verified
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ELISA Verified
Not Verified
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ChIP Verified
Not Verified
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Dot Blot Verified
Not Verified
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Flow Cytometry Verified
Not Verified
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
B2M
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Short name
Anti-Beta-2 Microglobulin Antibody (B2M/961)
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Technique
Antibody, anti-, anti, antibody to, antibodies, antibodies against human proteins, antibodies for
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Isotype
Mouse IgG2b, kappa
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Alternative name
Antibody toBeta-2 Microglobulin (antibody to-) (beta-2-microglobulin/961)
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Alternative technique
antibodies
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Alternative to gene target
beta-2-microglobulin, B2M and IDBG-9902 and ENSG00000166710 and 567, identical protein binding, Extracellular, B2m and IDBG-202736 and ENSMUSG00000060802 and 12010, B2M and IDBG-646936 and ENSBTAG00000012330 and 280729,783680
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Gene info
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Identity
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Gene
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Long gene name
beta-2-microglobulin
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GenBank acession
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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