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Human Recombinant Beta-Enolase

Human Recombinant Beta-Enolase is available 1 time from Biovision labs

6364-50 | Human Recombinant Beta-Enolase size: 50 ug | 553.24 USD

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Synonyms Human Recombinant Beta-Enolase
Alternative_names ENO3, MSE
Description A metalloenzyme responsible for conversion of 2-PG to PEP
Recombinant Yes
Source E. Coli
Purity by SDS-PAGE ≥95%
Assay SDS-PAGE
Biological activity Specific activity: >1.5 units/ml.
Results >1.5 units/ml
Unit Definition One unit will convert 1.0 µmole of 2-phosphoglycerate to phosphoenol pyruvate per minute at pH 7.5 at 25°C.
Molecular Weight 49 kDa (454 aa, 1-434 aa + NT His Tag)
Storage Temp. -20°C
Shipping gel pack
Shelf Life 12 months
Concentration 0.5 mg/ml
Appearance Liquid
Physical form description 0.5 mg/ml solution in 20 mM Tris-HCl (pH 8.0) containing 1 mM DTT, 20% glycerol and 0.1 mM NaCl.
Background Information Beta-enolase, also known as ENO3, is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. ENO3 play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. Mutations in its gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Recombinant human ENO3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Amino acid sequence MGSSHHHHHH SSGLVPRGSH MAMQKIFARE ILDSRGNPTV EVDLHTAKGR FRAAVPSGAS TGIYEALELR DGDKGRYLGK GVLKAVENIN STLGPALLQK KLSVADQEKV DKFMIELDGT ENKSKFGANA ILGVSLAVCK AGAAEKGVPL YRHIADLAGN PDLILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFKE AMRIGAEVYH HLKGVIKAKY GKDATNVGDE GGFAPNILEN NEALELLKTA IQAAGYPDKV VIGMDVAASE FYRNGKYDLD FKSPDDPARH ITGEKLGELY KSFIKNYPVV SIEDPFDQDD WATWTSFLSG VNIQIVGDDL TVTNPKRIAQ AVEKKACNCL LLKVNQIGSV TESIQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL MRIEEALGDK AIFAGRKFRN PKAK
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetBeta-Enolase
Short name Recombinant Beta-Enolase
Technique Recombinant
Species Human
Alternative name H. sapiens recombinant b-Enolase
Alternative technique rec
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