mTOR Antibody

• Catalog number
  3786-100
• Price
  Please ask
• Size
  100 μl

• Antibody Target
  mTOR
• Target Alternative Name
  FRAP1, FK506 binding protein 12-rapamycin associated protein 1; FRAP2, FK506 binding protein 12-rapamycin associated protein 2; RAFT1, RAPT1
• Antibody Type
  Polyclonal
• Species Reactivities
  Human, mouse, rat
• Immunogen Sequence
  Synthetic peptide of human mTOR
• Accession
  NP_004949.1
• Gene Id
  2475
• Appearance
  Colorless liquid
• Form
  Liquid
• Formulation
  100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
• Purification
  Affinity purified
• Application
  Western blot
• Application And Usages
  Western blotting (1:250 – 1:2500). Rat liver tissue lysate can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the mTOR of human, mouse, and rat origins. Reactivity to other species has not been tested.
• Description
  The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy.
• Storage Condition
  -20 °C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• Usage
  For Research Use Only! Not to be used in humans.
• Handling
  The antibody solution should be gently mixed before use.
• Western Blot Verified
  Verified
• Immunocytochemistry Verified
  Not Verified
• Immunofluorescence Verified
  Not Verified
• Immunoprecipitation Verified
  Not Verified
• FACS Verified
  Not Verified
• ELISA Verified
  Not Verified
• ChIP Verified
  Not Verified
• Flow Cytometry Verified
  Not Verified
• Data Sheet Link
• Properties
  If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  mTOR  
• Gene symbol
  MTOR-AS1, RPTOR, RICTOR
• Short name
  mTOR Antibody
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Mammalian target on rapamycin (antibody to-)
• Alternative technique
  antibodies
• Alternative to gene target
  mechanistic target of rapamycin (serine/threonine kinase), FRAP and FRAP1 and FRAP2 and RAFT1 and RAPT1, MTOR and IDBG-89258 and ENSG00000198793 and 2475, transferase activity, nuclei, Mtor and IDBG-204403 and ENSMUSG00000028991 and 56717, MTOR and IDBG-632731 and ENSBTAG00000015325 and 100139219

Gene info

• Identity
  HGNC:40242
• Gene
  MTOR-AS1
• Long gene name
  MTOR antisense RNA 1
• Synonyms gene name
  • MTOR antisense RNA 1 (non-protein coding)
• Locus
  1p36.22
• Discovery year
  2011-05-18
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000002003

Gene info

• Identity
  HGNC:30287
• Gene
  RPTOR
• Long gene name
  regulatory associated protein of MTOR complex 1
• Synonyms gene name
  • regulatory associated protein of MTOR, complex 1
• Synonyms
  • KOG1
  • Mip1
  • KIAA1303
  • raptor
• Synonyms name
  • regulatory associated protein of mTOR
• Locus
  17q25.3
• Discovery year
  2009-05-29
• Entrez gene record
  57521
• Pubmed identfication
  • 10718198
  • 12150926
• RefSeq identity
  • NM_020761
• Classification
  • WD repeat domain containing
  • MTOR complex 1
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000177622

Gene info

• Identity
  HGNC:28611
• Gene
  RICTOR
• Long gene name
  RPTOR independent companion of MTOR complex 2
• Synonyms gene name
  • RPTOR independent companion of MTOR, complex 2
• Synonyms
  • MGC39830
  • AVO3
  • PIA
  • KIAA1999
• Synonyms name
  • rapamycin-insensitive companion of mTOR
  • pianissimo
• Locus
  5p13.1
• Discovery year
  2009-05-29
• Entrez gene record
  253260
• Pubmed identfication
  • 15268862
• RefSeq identity
  • NM_152756
• Classification
  • MTOR complex 2
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000162037

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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