mTOR Antibody
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Catalog number3786-100
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PricePlease ask
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Size100 μl
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Antibody TargetmTOR
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Target Alternative NameFRAP1, FK506 binding protein 12-rapamycin associated protein 1; FRAP2, FK506 binding protein 12-rapamycin associated protein 2; RAFT1, RAPT1
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Antibody TypePolyclonal
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Species ReactivitiesHuman, mouse, rat
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Immunogen SequenceSynthetic peptide of human mTOR
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AccessionNP_004949.1
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Gene Id2475
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AppearanceColorless liquid
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FormLiquid
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Formulation100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.
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PurificationAffinity purified
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ApplicationWestern blot
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Application And UsagesWestern blotting (1:250 – 1:2500). Rat liver tissue lysate can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the mTOR of human, mouse, and rat origins. Reactivity to other species has not been tested.
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DescriptionThe mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1. These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy.
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Storage Condition-20 °C
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Shipping Conditiongel pack
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Shelf Life12 months
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UsageFor Research Use Only! Not to be used in humans.
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HandlingThe antibody solution should be gently mixed before use.
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Western Blot VerifiedVerified
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Immunocytochemistry VerifiedNot Verified
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Immunofluorescence VerifiedNot Verified
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Immunoprecipitation VerifiedNot Verified
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FACS VerifiedNot Verified
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ELISA VerifiedNot Verified
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ChIP VerifiedNot Verified
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Flow Cytometry VerifiedNot Verified
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PropertiesIf you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolMTOR-AS1, RPTOR, RICTOR
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Short namemTOR Antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameMammalian target on rapamycin (antibody to-)
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Alternative techniqueantibodies
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Alternative to gene targetmechanistic target of rapamycin (serine/threonine kinase), FRAP and FRAP1 and FRAP2 and RAFT1 and RAPT1, MTOR and IDBG-89258 and ENSG00000198793 and 2475, transferase activity, nuclei, Mtor and IDBG-204403 and ENSMUSG00000028991 and 56717, MTOR and IDBG-632731 and ENSBTAG00000015325 and 100139219
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Gene info
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Identity
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Gene
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Long gene nameMTOR antisense RNA 1
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Synonyms gene name
- MTOR antisense RNA 1 (non-protein coding)
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Locus
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Discovery year2011-05-18
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameregulatory associated protein of MTOR complex 1
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Synonyms gene name
- regulatory associated protein of MTOR, complex 1
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Synonyms
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Synonyms name
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Locus
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Discovery year2009-05-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- WD repeat domain containing
- MTOR complex 1
- Armadillo like helical domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameRPTOR independent companion of MTOR complex 2
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Synonyms gene name
- RPTOR independent companion of MTOR, complex 2
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Synonyms
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Synonyms name
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Locus
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Discovery year2009-05-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MTOR complex 2
- Armadillo like helical domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data