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Human Endothelin ELISA Kit

Human Endothelin ELISA Kit is available 4 times from Sab elisa kit labs

EK1832 | Human Endothelin-1 ELISA Kit size: 96-wells plate (breakable strips) | 624.69 USD

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EK4305 | Human Endothelin-2 ELISA Kit size: 96-wells plate (breakable strips) | 643.86 USD

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EK4302 | Human Endothelin-3 ELISA Kit size: 96-wells plate (breakable strips) | 643.86 USD

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EK4928 | Human Endothelin-converting enzyme 1 ELISA Kit size: 96-wells plate (breakable strips) | 781.43 USD

Catalog number EK4928
Price 781.43 USD
Size 96-wells plate (breakable strips)
Long name ELISA test kit for Human Endothelin-converting enzyme 1
Synonym name Homo sapiens Endothelin-converting enzyme 1 ELISA Kit
General description This is a ready to use kit for performing ELISA tests for detection of Endothelin-converting enzyme 1 in samples from Human
Antigen Endothelin-converting enzyme 1
Sample types Our Endothelin-converting enzyme 1 ELISA kit has been proven to work and successfully detect Endothelin-converting enzyme 1 in Human
Kit contents ELISA test kit for Human Endothelin-converting enzyme 1Assay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
Shipping, storage, shelf life The Endothelin-converting enzyme 1 ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Human Endothelin-converting enzyme 1 ELISA Kit is 6 months after delivery.
Tips Due to transportation and handling small amounts of the liquid components of the Homo sapiens Endothelin-converting enzyme 1 ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.
Gene targetEndothelin-converting
Short name Endothelin-converting enzyme 1 ELISA
Technique ELISA kit, Enzyme, ELISA
Alternative name H. sapiens Endothelin-converting enzyme 1 Enzyme-linked immunosorbent assay reagent
Alternative technique enzymes
1. Gene info
Identity 3146
Gene ECE1
Long gene name endothelin converting enzyme 1
Synonyms gene
  • ECE
GenBank acession
  • D49471
Locus 1p36.12
Discovery year 1995-12-08
Entrez gene record 1889
Pubmed identfication
  • 7805846
  • 7864876
  • 17592116
RefSeq identity
  • NM_001397
Classification
  • M13 metallopeptidases
Havana BLAST/BLAT OTTHUMG00000002625
Product images
    #Human#.jpg
Species reactivity Human
Detection wavelengt 450 nm
Assay procedure 1-4h
Summary of the prodecure 1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
Applications Enzyme Linked Immunosorbent Assay
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Human
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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