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Human CellExp™ TIMP1, human recombinant

Human CellExp™ TIMP1, human recombinant is available 1 time from Biovision labs

7440-10 | Human CellExp™ TIMP1, human recombinant size: 10 ug | 315.87 USD

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Synonyms TIMP1, TIMP-1, CLGI, EPA, EPO, FLJ90373, HCI, TIMP, TIMP metallopeptidase inhibitor 1
Alternative_names TIMP1, TIMP-1, CLGI, EPA, EPO, FLJ90373, HCI, TIMP, TIMP metallopeptidase inhibitor 1
Description A natural inhibitor of the matrix metalloproteinases (MMPs)
Recombinant Yes
Source HEK293 cells
Purity by SDS-PAGE ≥95%
Assay SDS-PAGE
Biological activity Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.The IC50 value is approximately 2.5 nM.
Results Measured by its ability to inhibit human MMP2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2.The IC50 value is approximately 2.5 nM.
Molecular Weight This protein contains C-terminal polyhistidine tag and has a calculated MW of 24 kDa. DTT-reduced protein migrates as 28-32 kDa polypeptide in SDS-PAGE.
Storage Temp. -20°C
Shipping Gel Pack
Shelf Life 12 months
Appearance Lyophilized
Physical form description Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Reconstitution Instructions Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Background Information TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein a member of the TIMP family. There are four members of the family, TIMP1, TIMP2, TIMP3 and TIMP4. TIMP1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetCellExp™ TIMP1
Short name CellExp™ TIMP1, recombinant
Technique Recombinant
Species Humans
Alternative name H. sapiens CellExp™ TIMP1, H. sapiens recombinant
Alternative technique rec
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