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Anti DNA Polymerase ALEXA Fluor 594

Anti DNA Polymerase ALEXA Fluor 594 is available 6 times from Bioss polyclonal antibodies labs

GENTObs-8525R-A594 | Anti-DNA Polymerase beta (Polyclonal), ALEXA Fluor 594 size: 100 microliters | 568.71 USD

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GENTObs-13017R-A594 | Anti-DNA Polymerase gamma (Polyclonal), ALEXA Fluor 594 size: 100 microliters | 568.71 USD

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GENTObs-13018R-A594 | Anti-DNA Polymerase iota (Polyclonal), ALEXA Fluor 594 size: 100 microliters | 568.71 USD

Catalog number GENTObs-13018R-A594
Price 568.71 USD
Size 100 microliters
Target Protein/Peptide DNA Polymerase iota
Specificity This antibody reacts specifically with DNA Polymerase iota
Subcellular locations N/A
Antigen Source KLH conjugated synthetic peptide derived from human DNA Polymerase iota
Gene ID 11201
Swiss Prot N/A
Background information DNA polymerase activity is essential for replication, repair, recombination and mutagenesis. DNA polymerases can often bypass DNA lesions that block DNA replication, thereby allowing the replication of damaged DNA. One such DNA polymerase is the distributive enzyme DNA Pol i, which is encoded by the POLI gene. POLI is located on human chromosome 18q21.2, a region often implicated in the etiology of many human cancers. At thymine templates, DNA Pol i is highly error-prone when replicating undamaged DNA in that it favors the misincorporation of guanine over the correct nucleotide, adenosine. DNA Pol i also promotes the replication of damaged DNA by misincorporating deoxynucleotides opposite DNA lesions. DNA Pol i acts sequentially with DNA Pol Ω, which is essential for damage-induced mutagenesis, to complete the DNA lesion bypass. Therefore, replication involving DNA Pol i is likely to be highly mutagenic.
Synonyms DNA polymerase iota; Eta 2; Eta2; POLI; POLI_HUMAN; Polymerase DNA directed iota; RAD 30B; RAD30 homolog B; RAD30B; RAD3OB.
Also known as DNA Polymerase iota Polyclonal Antibody
Other name Anti-DNA Polymerase iota Polyclonal
Properties For facs or microscopy Alexa 1 conjugate.
Conjugation Alexa Fluor,ALEXA FLUOR® 594
Conjugated Alexa conjugate 1
Description This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Group Polyclonals and antibodies
About Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Gene targetDNA Polymerase iota
Short name Anti-DNA Polymerase iota
Technique Polyclonal, anti, dna
Alternative name DNA Polymerase iota Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated
Alternative technique polyclonals
1. Gene info
Identity 9413
Gene PRKDC
Long gene name protein kinase, DNA-activated, catalytic subunit
Synonyms gene
  • HYRC
  • HYRC1
Synonyms gene name
  • protein kinase, DNA-activated, catalytic polypeptide
Synonyms
  • DNPK1
  • p350
  • DNAPK
  • XRCC7
  • DNA-PKcs
  • DNAPKc
  • DNA-PKC
  • p460
Synonyms name
  • DNA-dependent protein kinase catalytic subunit
Locus 8q11.21
Discovery year 1993-11-09
Entrez gene record 5591
Pubmed identfication
  • 7638222
RefSeq identity
  • NM_001081640
Classification
  • Armadillo like helical domain containing
Havana BLAST/BLAT OTTHUMG00000164239
Locus Specific Databases
  • LRG_162
2. Gene info
Identity 9182
Gene POLI
Long gene name DNA polymerase iota
Synonyms gene
  • RAD3OB
  • RAD30B
Synonyms gene name
  • polymerase (DNA directed) iota
  • polymerase (DNA) iota
Locus 18q21.2
Discovery year 2000-01-24
Entrez gene record 11201
Pubmed identfication
  • 17609217
RefSeq identity
  • NM_007195
Classification
  • DNA polymerases
Havana BLAST/BLAT OTTHUMG00000132704
Product images
    Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes;  Blocking buffer (normal goat serum) at 37°C for 20min; An

GENTObs-13019R-A594 | Anti-DNA Polymerase Kappa/POLK, ALEXA Fluor 594 size: 100 microliters | 568.71 USD

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GENTObs-13020R-A594 | Anti-DNA Polymerase lambda, ALEXA Fluor 594 size: 100 microliters | 568.71 USD

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GENTObs-13022R-A594 | Anti-DNA Polymerase theta/POLH, ALEXA Fluor 594 size: 100 microliters | 568.71 USD

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Type Conjugated Primary Antibody
Conjugated with ALEXA FLUOR® 594
Host organism Rabbit (Oryctolagus cuniculus)
Modification No modification has been applied to this antibody
Modification site None
Clonality Polyclonal Antibody
Clone Polyclonal Antibodies
Concentration 1ug per 1ul
Applications IF(IHC-P)
Applications with corresponding dilutions IF(IHC-P)(1:50-200)
Cross reactive species Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Cross Reactive Species details No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Purification method Purified by Protein A.
Storage Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Excitation emission 590nm/617nm
Advisory Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Isotype Immunoglobulin G (IgG)
Label ALEXA FLUOR 594
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Anti-DNA Polymerase iota | Technique alternative | 010111877
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