Bovine IgG Fc FITC

  • Catalog number
    65C-CH0105
  • Price
    Please ask
  • Size
    1 mg
  • Category
    Immunoglobulins
  • Antibody Subtype
    Conjugated Immunoglobulins
  • Area of research
    Immunology
  • Reactivity
    Bovine
  • Conjugate Tag
    FITC
  • Method of Purification
    Bovine IgG (Fc) (FITC) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
  • Form Buffer
    Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, and 0.01% NaN3.
  • Storage
    Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
  • Shipping conditions
    Blue Ice
  • Tested for
    IF
  • Usage Recommendations
    Optimal conditions to be determined by end user
  • URL
  • Properties
    This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • About
    Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
  • Gene target
    -
  • Gene symbol
    FCGBP, FCGR1A, FCRLB
  • Short name
    IgG Fc FITC
  • Technique
    FITC, IgG, FC, Fluorescein, IgGs
  • Isotype
    IgG, IgG
  • Label
    FITC
  • Species
    Bovine, Bos Taurus genes are available form the Bovine Genome database.
  • Alternative name
    Purified Bovine IgG (Fc) FITC conjugate
  • Alternative technique
    fluorescine, immunoglobulins
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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