PEDF, human recombinant

• Catalog number
  4988-1000
• Price
  Please ask
• Size
  1 mg

• Synonyms
  Pigment epithelium-derived factor, PEDF, Serpin-F1, SerpinF1, EPC-1, EPC1, PIG35
• Alternative_names
  Pigment epithelium-derived factor, PEDF, Serpin-F1, SerpinF1, EPC-1, EPC1, PIG35
• Description
  A multifunctional protein with anti-angiogenic, anti-tumorigenic, and neurotrophic functions
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Purity
  ≥95%
• Biological activity
  Not available at this time
• Molecular Weight
  44.5 kDa
• Storage Temp
  -20°C
• Shipping
  Blue ice
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  The sterile filtered concentrated (1 mg/ml) protein solution was lyophilized with 20 mM PBS & 150 mM NaCl pH-7.4.
• Reconstitution Instructions
  The sterile filtered concentrated (1 mg/ml) protein solution was lyophilized with 20 mM phosphate buffer & 150 mM NaCl pH-7.4.
• Background Information
  PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. PEDF is a 50,000 dalton glycoprotein created and secreted in many tissues all the way through the body. A key component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. Additionally, PEDF is capable to inhibit the activity of angiogenic factors such as VEGF and FGF-2. The recognition of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. The unique range of PEDF activities associate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF in addition has the potential to be functional in the treatment of various angiogenesis-related diseases including a number of cancers. Recombinant human PEDF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 400 amino acids and having a molecular mass of 44.5 kDa.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  PEDF  
• Gene symbol
  SERPINF1
• Short name
  PEDF, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  PEDF, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:8824
• Gene
  SERPINF1
• Long gene name
  serpin family F member 1
• Synonyms gene
  • PEDF
• Synonyms gene name
  • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
  • pigment epithelium-derived factor
• Synonyms
  • EPC-1
  • PIG35
• Synonyms name
  • alpha-2 antiplasmin
  • proliferation-inducing protein 35
• GenBank acession
  • M76979
• Locus
  17p13.3
• Discovery year
  1993-05-18
• Entrez gene record
  5176
• Pubmed identfication
  • 8434014
  • 24172014
• RefSeq identity
  • NM_002615
• Classification
  • Serpin peptidase inhibitors
• VEGA ID
  OTTHUMG00000090571
• Locus Specific Databases
  • SERPINF1 variant database

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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