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Rabbit F ab 2 fragment conjugated

Rabbit F ab 2 fragment conjugated is available 3 times from Agisera labs

AS10 997 | Rabbit, F(ab)'2 fragment, Biotin conjugated size: 0.5 mg | 414.80 USD

Catalog number AS10 997
Supplier agisera
Price414.80 USD
Size 0.5 mg
Available ordering format Liquid
Purification Affinity purified goat IgG, F(ab)'2 fragment
How to reconstitute See included datasheet or contact our support service
Storage condition Store at 2-8°C. Shelf life of this product is one year from date of receipt.
Connected products other biotinylated secondary antibodies to goat IgG (H&L)
Recommended dilutions for use Needs to be determined by end user for all immunoassay applications.
Scientific context Rabbit anti-goat IgG (H&L), F(ab)'2 fragment is a biotinylated secondary antibody which binds to goat IgG (H&L), F(ab)'2 fragment Antibody purity is > 90% based on SDS-PAGE. Antibody solution may contain small amounts of intact IgG.
Notes This antibody reacts with the heavy chains on goat IgG and with the light chains on all goat immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin goat serum proteins based on immunoelectrophoresis.Antibody is supplied in 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free and 0.1 % (v/v) Kathon CG is used as preservative. Use of sodium azide will inhibit enzymatic activity of horseradish peroxidase.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation Biotinylated
About Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name Oryctolagus cuniculus
Gene targetF(ab)'2 conjugated
Short name Rabbit, F(ab)'2 conjugated
Isotype Antibody fragments and scFv fragments, single chain variable fragments can be E. coli expressed. Fragment peptides for polyclonals give monoclonal like antibodies as the epitope is very small. IgG Fc and(ab)2 fragments are small and stable subunits of the antibody obtained by enzymatic digestion. Complement fragments, bp DNA fragments are common.
Label Biotin
Alternative name production species: rabbit, F(Antibody)'2 fragment, biotinilated coupled
MeSH Data
NameBiotin
Concept
Registry number:6SO6U10H04
Scope note:A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
CASN1 number:1H-Thieno(3,4-d)imidazole-4-pentanoic acid, hexahydro-2-oxo-, (3aS-(3aalpha,4beta,6aalpha))-
Tree numbers
  • D03.383.129.308.080
  • D08.211.096
Additional information

AS10 1045 | Rabbit, F(ab)'2 fragment, HRP conjugated size: 0.5 mg | 414.80 USD

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AS10 980 | Rabbit, F(ab)'2 fragment, TRITC conjugated size: 0.5 mg | 414.80 USD

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Clonality Polyclonal
TAIR number Refer to NCBI
No reactivity to be determined
Raised in Rabbit
Verified reactivity to be determined
Molecular weight (expected | аpparent) see datasheet
References Refer to PubMed
Immunogen to be determined
Protein number Refer to NCBI
Supplementary information Contact Gentaur support center at info@gentaur.com
Clone Polyclonal
Verified applications Contact Gentaur support center
Possible reactivity to be determined
Technique Rabbit
Host Rabbit
Alternative technique rabbit-anti
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