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Category
Primary Antibodies
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Long description
Integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide), also known as ITGAL, is a Human gene which functions in the immune system. It is involved in cellular adhesion and costimulatory signaling. ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 is part of the family of leukocyte integrins that are recognised by their common β-chains (CD18). LFA-1 also has a distinct α-chain (CD11a). LFA-1 is expressed on lymphocytes, monocytes and granulocytes; it has been shown that LFA-1 contributes to the adhesion reaction of these cells. Blocking experiments with anti-LFA monoclonal antibodies demonstRated that LFA-1 inhibits the adhesion step effector and target cells in cytotoxic T lymphocyte, natural killer and lectin dependent cytotoxicity. LFA-1 is involved in recruitment to the site of infection. It binds to ICAM-1 on antigen-presenting cells and functions as an adhesion molecule. LFA-1 is the first to bind T-cells to antigen-presenting cells and initially binds weakly. A signal from the T-cell receptor and/or the cytokine receptor changes the conformation and prolongs the cell contact, allowing the T-cell to prolifeRate. The antibody was selected for inhibition of T-cell mediated killing.
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Antibody come from
NKI(SPV)-L15 was obtained by immunization of BALB/c mice with cells of the T4+T8- cytotoxic T lymphocyte clone HG-38; the antibodies were selected for inhibition of T cell mediated killing by screening of the hybridoma supernatants 10-14 days after the fusion.
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Other description
Each vial contains 100 ml 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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Clone
NKI(SPV)-L15
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Antigen antibody binding interaction
Mouse anti CD11a inhibitor Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
The monoclonal antibody NKI(SPV)-L15 reacts with the alpha chain of LFA-1, the CD11a/CD18 integrin heterodimer present on Human T- and B-lymphocytes, granulocytes and monocytes. The antibody was selected for inhibition of T-cell mediated killing.
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Research interest
CD markers,Cell adhesion
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Application
Flow Cytometry,Immunoprecipitation,Western blotting
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Antibody s suited for
NKI(SPV)-L15 is useful for immunoprecipitation, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titration.
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Storage
contact us for more details about storage and/or shipping
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Relevant references
Cytoplasmic Tails of β1, β2 and β7 integrins differentially regulate LFA-1 function in K562 cells. M.Lub, S.J van Vliet, S.P.M.A Oomen, R.A Pieters, M. Robinson, C.G Figdor, Y van Kooyk. Mol. Biol of the Cell, Vol 8, 719-728, April 1997.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Tissue, pathway, proteinase, peptidase, protease ,acrosin, lipoprotein, activator, caspase, trypsin, papain, esterase inhibitors are proteins or receptor ligands or receptor antagonists that bind to an enzyme receptor and decreases its activity. Since blocking an enzyme's activity can kill a pathogen or correct a metabolic imbalance, many drugs are enzyme inhibitors. Not all receptor antagonist that bind to enzymes are inhibitors; enzyme activator ligands or agonists bind to enzymes and increase their enzymatic activity, while enzyme substrates bind and are converted to products in the normal catalytic cycle of the enzyme.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
