SCF, rat recombinant

• Catalog number
  4329-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
• Alternative_names
  Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.
• Description
  A cytokine playing an important role in hematopoiesis, spermatogenesis, & melanogenesis
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥98%
• Assay
  SDS-PAGE
• Purity
  ≥98%
• Biological activity
  The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of human MO7e cells is 1 x 10⁵ units/mg.
• Molecular Weight
  18.4 kDa
• Storage Temp
  -20°C
• Shipping
  Blue ice
• Shelf Life
  12 months
• Appearance
  Lyophilized protein
• Physical form description
  Lyophilized from 20 mM Tris, pH 7.5
• Reconstitution Instructions
  Reconstitute with sterile 10 mM acetic acid to a concentration of 0.1 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
• Background Information
  Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures. Recombinant rat SCF is an 18.4 kDa protein containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• About
  Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  SCF  
• Gene symbol
  KITLG
• Short name
  SCF, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Host
  Rat
• Species
  Rat, Rats
• Alternative name
  SCF, rat Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:6343
• Gene
  KITLG
• Long gene name
  KIT ligand
• Synonyms gene
  • MGF
• Synonyms
  • SCF
  • SF
  • Kitl
  • KL-1
  • FPH2
  • SLF
  • DFNA69
• Synonyms name
  • mast cell growth factor
  • stem cell factor
  • steel factor
  • familial progressive hyperpigmentation 2
• GenBank acession
  • M59964
• Locus
  12q21.32
• Discovery year
  1991-06-04
• Entrez gene record
  4254
• Pubmed identfication
  • 2208279
  • 1707188
  • 19375057
  • 26522471
• RefSeq identity
  • NM_003994
• VEGA ID
  OTTHUMG00000169888
• Locus Specific Databases
  • LRG_1379

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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