AKR1B10, human recombinant

  • Catalog number
    6339-1000
  • Price
    Please ask
  • Size
    1 mg
  • Synonyms
    Aldo-keto reductase family 1, member B10, AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HIS.
  • Alternative_names
    Aldo-keto reductase family 1, member B10, AKR1B11, AKR1B12, ALDRLn, ARL-1, ARL1, HIS, HIS.
  • Description
    Involved in in the detoxification of aldehydes and ketones
  • Recombinant
    Yes
  • Source
    E. coli
  • Purity by SDS PAGE
    ≥98%
  • Assay
    SDS-PAGE
  • Biological activity
    The specific activity of AKR1B10 with 5 mM glyceraldehyde is approximately ~100 mU/mg. (Specific activity is calculated by the decrease of absorption at 340 nm in presence of 2-10 µg AKR1B10, 5mM glyceraldehyde, 200 µM NADPH in 0.1 M sodium phosphate buffer, pH 7.5).
  • Results
    ~100 mU/mg
  • Unit Definition
    One unit is the amount of enzyme that oxidizes 1.0 µmole of NADPH to NADP+ per min. at pH 7.5 at 25°C.
  • Molecular Weight
    36 kDa
  • Storage Temp
    -20°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Concentration
    1 mg/ml
  • Appearance
    Liquid
  • Physical form description
    1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol.
  • Background Information
    AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications. It has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer.
  • Amino acid sequence
    MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
    AKR1B10  
  • Gene symbol
    AKR1B10
  • Short name
    AKR1B10, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    AKR1B10, H. sapiens Rec.
  • Alternative technique
    rec
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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