Recombinant Human Histone Chaperone ASF1A/ASF1A Protein(C-6His, N-T7 tag)

• Catalog number
  PKSH032549-10µg
• Price
  Please ask
• Size
  10µg

• Synonym
  Histone Chaperone ASF1A; Anti-Silencing Function Protein 1 Homolog A; hAsf1; hAsf1a; CCG1-Interacting Factor A; CIA; hCIA; ASF1A
• Activity
  NA
• Sequence
  Met1-Met204
• Fusion tag
  C-6His
• Accession
  Q9Y294
• Expressed Host
  E.coli
• Shipping
  The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
• Purity
  ＞95% as determined by reducing SDS-PAGE.
• Endotoxin
  ＜1.0 EU per µg as determined by LAL test.
• Stability and Storage
  Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
• Mol Mass
  25.4 kDa
• AP Mol Mass
  22 kDa
• Formulation
  Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl, 1mM DTT, 150mM NaCl, pH 8.0.
• Background
  Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many cells and tissues, interacting with histones H3 and H4. ASF1A cooperates with Chromatin Assembly Factor 1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. In addition, ASF1A is necessary for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
• Technical data
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Histone   Chaperone   ASF1A/ASF1A   Protein   C-6His   N-T7   tag  
• Gene symbol
  ASF1A, GALNT7, GH1, POLM, B4GALT7, NRAS, SDC3, PIGN, WASL, MAP6
• Short name
  Recombinant Histone Chaperone ASF1A/ASF1A Protein(C-6His, N-T7 tag)
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
• Label
  tag
• Species
  Human, Humans
• Alternative name
  Rec. H. sapiens Histone Chaperone ASF1A/ASF1A Protein(C-6His, N-T7 detection labelled)
• Alternative technique
  rec

Gene info

• Identity
  HGNC:20995
• Gene
  ASF1A
• Long gene name
  anti-silencing function 1A histone chaperone
• Synonyms gene name
  • ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
• Synonyms
  • DKFZP547E2110
  • CIA
• GenBank acession
  • AF279306
• Locus
  6q22.31
• Discovery year
  2003-05-01
• Entrez gene record
  25842
• Pubmed identfication
  • 10810093
  • 11042152
• RefSeq identity
  • NM_014034
• VEGA ID
  OTTHUMG00000015470

Gene info

• Identity
  HGNC:4129
• Gene
  GALNT7
• Long gene name
  polypeptide N-acetylgalactosaminyltransferase 7
• Synonyms gene name
  • UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 7 (GalNAc-T7)
• Synonyms
  • GALNAC-T7
• Synonyms name
  • polypeptide GalNAc transferase 7
• GenBank acession
  • AJ002744
• Locus
  4q34.1
• Discovery year
  1999-09-16
• Entrez gene record
  51809
• Pubmed identfication
  • 10544240
• RefSeq identity
  • NM_017423
• Classification
  • Polypeptide N-acetylgalactosaminyltransferases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000160817

Gene info

• Identity
  HGNC:4261
• Gene
  GH1
• Long gene name
  growth hormone 1
• Synonyms
  • GH-N
  • GHN
  • GH
  • hGH-N
• Synonyms name
  • pituitary growth hormone
  • somatotropin
• GenBank acession
  • M13438
• Locus
  17q23.3
• Discovery year
  2001-06-22
• Entrez gene record
  2688
• Pubmed identfication
  • 6306568
• RefSeq identity
  • NM_000515
• Classification
  • Growth hormone family
• VEGA ID
  OTTHUMG00000172293

Gene info

• Identity
  HGNC:9185
• Gene
  POLM
• Long gene name
  DNA polymerase mu
• Synonyms gene name
  • polymerase (DNA directed), mu
  • polymerase (DNA) mu
• Synonyms
  • Tdt-N
• Synonyms name
  • Pol iota
• GenBank acession
  • AF176097
• Locus
  7p13
• Discovery year
  2000-02-16
• Entrez gene record
  27434
• Pubmed identfication
  • 10982892
• RefSeq identity
  • NM_013284
• Classification
  • DNA polymerases
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000155353

Gene info

• Identity
  HGNC:930
• Gene
  B4GALT7
• Long gene name
  beta-1,4-galactosyltransferase 7
• Synonyms gene name
  • xylosylprotein beta 1,4-galactosyltransferase, polypeptide 7
• Synonyms
  • XGALT-1
  • beta4Gal-T7
• Synonyms name
  • galactosyltransferase I
• GenBank acession
  • AB028600
• Locus
  5q35.3
• Discovery year
  1999-12-07
• Entrez gene record
  11285
• Pubmed identfication
  • 10438455
  • 10473568
  • 15211654
  • 24052259
• RefSeq identity
  • NM_007255
• Classification
  • Beta 4-glycosyltransferases
• VEGA ID
  OTTHUMG00000130851

Gene info

• Identity
  HGNC:7989
• Gene
  NRAS
• Long gene name
  NRAS proto-oncogene, GTPase
• Synonyms gene name
  • neuroblastoma RAS viral (v-ras) oncogene homolog
  • neuroblastoma RAS viral oncogene homolog
• Synonyms
  • N-ras
• GenBank acession
  • BC005219
• Locus
  1p13.2
• Discovery year
  2001-06-22
• Entrez gene record
  4893
• Pubmed identfication
  • 6705568
  • 11524732
• RefSeq identity
  • NM_002524
• Classification
  • RAS type GTPase family
• VEGA ID
  OTTHUMG00000012059
• Locus Specific Databases
  • NRASbase: Mutation registry for autoimmune lymphoproliferative syndrome type IV
  • LRG_92

Gene info

• Identity
  HGNC:10660
• Gene
  SDC3
• Long gene name
  syndecan 3
• Synonyms gene name
  • syndecan 3 (N-syndecan)
• Synonyms
  • N-syndecan
  • SYND3
• Synonyms name
  • syndecan proteoglycan 3
• GenBank acession
  • AF248634
• Locus
  1p35.2
• Discovery year
  1994-05-16
• Entrez gene record
  9672
• Pubmed identfication
  • 1556152
  • 11527150
• RefSeq identity
  • NM_014654
• Classification
  • Syndecans
• VEGA ID
  OTTHUMG00000043646

Gene info

• Identity
  HGNC:8967
• Gene
  PIGN
• Long gene name
  phosphatidylinositol glycan anchor biosynthesis class N
• Synonyms gene name
  • phosphatidylinositol glycan, class N
• Synonyms
  • MCD4
  • PIG-N
• GenBank acession
  • AF109219
• Locus
  18q21.33
• Discovery year
  2000-05-11
• Entrez gene record
  23556
• Pubmed identfication
  • 10069808
  • 10574991
• RefSeq identity
  • NM_176787
• Classification
  • Phosphatidylinositol glycan anchor biosynthesis
• VEGA ID
  OTTHUMG00000180098

Gene info

• Identity
  HGNC:12735
• Gene
  WASL
• Long gene name
  WASP like actin nucleation promoting factor
• Synonyms gene name
  • Wiskott-Aldrich syndrome-like
  • Wiskott-Aldrich syndrome like
• Synonyms
  • N-WASP
  • NWASP
  • WASPB
• GenBank acession
  • D88460
• Locus
  7q31.32
• Discovery year
  1998-10-14
• Entrez gene record
  8976
• Pubmed identfication
  • 9422512
  • 9322739
  • 23527602
• RefSeq identity
  • NM_003941
• Classification
  • Wiskott-Aldrich Syndrome protein family
• VEGA ID
  OTTHUMG00000157346

Gene info

• Identity
  HGNC:6868
• Gene
  MAP6
• Long gene name
  microtubule associated protein 6
• Synonyms
  • KIAA1878
  • STOP
  • FLJ41346
  • MAP6-N
• GenBank acession
  • AK123340
• Locus
  11q13.5
• Discovery year
  1998-02-26
• Entrez gene record
  4135
• Pubmed identfication
  • 10516426
  • 12231625
• RefSeq identity
  • NM_033063
• VEGA ID
  OTTHUMG00000165343

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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