Nanog-TAT, human recombinant
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Catalog number7177-50
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PricePlease ask
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Size50 ug
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SynonymsHomeobox transcription factor Nanog
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Alternative_namesHomeobox transcription factor Nanog
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DescriptionA regulatory protein associated with undifferentiated pluripotent cells
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RecombinantYes
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SourceE. Coli
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Purity by SDS PAGE≥98%
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AssaySDS-PAGE
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Endotoxin Level< 0.1 ng/ug of protein (
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Molecular Weight36.2 kDa
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Storage Temp-20°C
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ShippingGel Pack
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Shelf Life12 months
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AppearanceLyophilized powder
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Physical form descriptionSterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Sodium Citrate pH 3.0 and 100 mM NaCl.
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Reconstitution InstructionsCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background InformationNanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which helps maintain pluripotency and self-renewal via a STAT3 independent pathway. The introduction of Nanog, along with Sox2, Oct4, Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human Nanog-TAT is a 36.2 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide.
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Amino acid sequenceMSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA ENSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDVGGYGR KKRRQRRR
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolTAT, TAT-AS1, HTATSF1, HTATSF1P1, TRI-TAT2-2, TRI-TAT1-1, TRI-TAT3-1, TRI-TAT2-3, TRI-TAT2-1
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Short nameNanog-TAT, recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative nameNanog-TAT, H. sapiens Rec.
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Alternative techniquerec
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Gene info
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Identity
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Gene
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Long gene nametyrosine aminotransferase
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Locus
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Discovery year2001-06-22
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameTAT antisense RNA 1
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GenBank acession
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Locus
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Discovery year2014-10-16
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Entrez gene record
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RefSeq identity
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Classification
- Antisense RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameHIV-1 Tat specific factor 1
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Synonyms gene name
- HIV TAT specific factor 1
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-07-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- RNA binding motif containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameHIV-1 Tat specific factor 1 pseudogene 1
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Synonyms gene
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Synonyms gene name
- HIV TAT specific factor 1 pseudogene
- HIV-1 Tat specific factor 1 pseudogene
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Synonyms
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Locus
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Discovery year2003-11-26
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Entrez gene record
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Ile (anticodon TAT) 2-2
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Synonyms gene
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Synonyms gene name
- transfer RNA isoleucine 2 (anticodon UAU)
- transfer RNA-Ile (TAT) 2-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Ile (anticodon TAT) 1-1
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Synonyms gene
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Synonyms gene name
- transfer RNA isoleucine 18 (anticodon UAU)
- transfer RNA-Ile (TAT) 1-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Ile (anticodon TAT) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA isoleucine 21 (anticodon UAU)
- transfer RNA-Ile (TAT) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Ile (anticodon TAT) 2-3
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Synonyms gene
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Synonyms gene name
- transfer RNA isoleucine 6 (anticodon UAU)
- transfer RNA-Ile (TAT) 2-3
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Ile (anticodon TAT) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA isoleucine 13 (anticodon UAU)
- transfer RNA-Ile (TAT) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data