Glycine N-methyltransferase, Human Recombinant

• Catalog number
  P1235-50
• Price
  Please ask
• Size
  50 ug

• Synonyms
  GNMT
• Alternative_names
  GNMT
• Description
  Regulate tissue concentration of AdoMet and metabolism of methionine.
• Recombinant
  Yes
• Source
  E.coli
• Purity by SDS PAGE
  > 95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per/ug
• Activity Specifications test method
  Measured by its ability to transfer 1.0 nmole of methyl group per minute at 37C.
• Biological activity
  Specific activity > 100 nmol/min/mg
• Results
  >100 nmol/min/mg
• Molecular Weight
  34.9 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol
• Background Information
  Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC). Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Glycine   N-methyltransferase  
• Gene symbol
  GNMT
• Short name
  Glycine N-methyltransferase, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Glycine N-methyltransferase, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:4415
• Gene
  GNMT
• Long gene name
  glycine N-methyltransferase
• GenBank acession
  • AF101475
• Locus
  6p21.1
• Discovery year
  2000-01-21
• Entrez gene record
  27232
• Pubmed identfication
  • 10843803
  • 9495250
• RefSeq identity
  • NM_018960
• Classification
  • 7BS small molecule methyltransferases
• VEGA ID
  OTTHUMG00000014712

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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