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Alternative name1
Anti-GOAT ANTI RAT IgG (H/L):Biotin
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Alternative name2
Caprine ANTI RAT IgG (H/L):Biotin
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Alternative name3
Caprinae ANTI Rattus norvegicus IgG (H/L):Biotin
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Alternative name4
IgG
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Alternative name5
N/A
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Other names
Ig gamma-2A chain C region; Ig gamma-2A chain C region; N/A; N/A; N/A
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Gene name
IgG
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Gene name synonims
N/A
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Other gene names
LOC679045; Igg-2a; N/A; N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
Polyclonal IgG
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Clone
Polyclonal antibody
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Host organism
Goat
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Species reactivity
Rat
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Specificity and cross reactivity
IgG This item is specific for the heavy and light chains of rat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, rat IgG and rat serum.
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Purification method
N/A
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Form Appearance
Biotin (Purified IgG conjugated to Biotin - lyophilised)
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Concentration
Ig concentration 2.0 mg/ml
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Storage and shipping
Prior to reconstitution store at +Store productone at +4 degrees Celsius.. After reconstitution store at +Store productone at +4 degrees Celsius. or productone should be stored at -20 degrees Celsius. if preferred. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should productone contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of reconstitution.
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Tested for
Immunohistology - Frozen (IHC), Western Blot (WB), ELISA (EIA)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Latin name
Capra aegagrus hircus, Rattus norvegicus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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French translation
anticorps
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Gene target
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Gene symbol
CALD1, FUT1, L3MBTL2
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Short name
GOAT ANTI IgG (H/L): Antibody
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Technique
Antibody, anti, Goat, IgG, antibody to, antibodies against human proteins, antibodies for, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, IgG
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Label
Biotin
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Species
Rat, Goats, Rats
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Alternative name
caprine antibody to RAT Immunoglobulin G (H/L):biotinilated (antibody to-)
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Alternative technique
antibodies, caprine, immunoglobulins
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