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Category
Primary Antibodies
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Long description
Orphan receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Expressed predominantly in the central nervous system, and at low levels in the lung, kidney, testis, ovary and eye. Belongs to the G-protein coupled receptor 1 family.
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Antibody come from
Synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho Thr292 of human MEK 1.
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Other description
Provided in HEPES (pH 7.5) solution containing 150 mM NaCl, 100 µg per ml BSA and 50% glycerol . Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti GPR3 Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot, Enzyme Immunoassay
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Antibody s suited for
Antibody should be used at a 1:1000 dilution to provide for 10 miniblots in Western blotting and dot blots. Antibody detects only phosphorylated protein and does not detect non-phosphorylated protein as shown by the lack of ability of a non-phospho peptide to block the antibody activity. Optimal concentration may be evaluated by serial dilutions.
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Storage
-80ºC
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Relevant references
1. Adams, J.P. and Sweatt, J.D. 'Molecular psychology: Roles for the ERK MAP kinase cascade in memory,' Annu. Rev. Pharmacol. Toxicol. 42, 135-163 (2002). _x000B__x000B_2. Ahn, N.G. 'The MAP kinase cascade. Discovery of a new signal transduction pathway,' Mol. Cell Biochem. 127-128, 201-209 (1993). _x000B__x000B_3. Ahn, N.G., et al., 'Identification of an activator of the microtubule-associated protein 2 kinases ERK1 and ERK2 in PC12 cells stimulated with nerve growth factor or bradykinin,' J. Neurochem. 59, 147-156 (1992). _x000B__x000B_4. Crews, C.M., Alessandrini, A. and Erikson, R.L. 'The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product,' Science 258, 478-480 (1992). _x000B__x000B_5. Mansour, S.J., et al. 'Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis.' J. Biochem. (Tokyo) 116, 304-314 (1994). _x000B__x000B_6. Park, S.H., Zarrinpar, A. and Lim, W.A., 'Rewiring MAP kinase pathways using alternative scaffold assembly mechanisms,' Science 299, 1061-1064 (2003)._x000B__x000B_7. Chong H, Vikis HG, Guan KL (2003) Mechanisms of regulating the Raf kinase family. Cellular Signalling 15:463- 469._x000B__x000B_8. Kyriakis JM, Brautigan DL, Ingebritsen TS, Avruch J (1991) pp54 Microtubule-associated protein-2 kinase requires both tyrosine and serine/threonine phosphorylation for activity. J Biol Chem 266:10043-10046._x000B__x000B_9. Seger R, Ahn NG, Boulton TG, Yancopoulos GD, Panayotatos N, Radziejewska E, Ericsson L, Bratlien RL, Cobb MH, Krebs EG (1991) Microtubule-associated protein 2 kinases, ERK1 and ERK2, undergo autophos- phorylation on both tyrosine and threonine residues: Implications for their mechanism of activation. Proc Natl Acad Sci USA 88:6142-6146.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
