Human CellExp™ FCER1A /FCE1A, human recombinant
-
Catalog number
7473-50
-
Price
Please ask
-
Size
50 ug
-
-
Synonyms
FCER1A, FCE1A, Ferris
-
Alternative_names
FCER1A, FCE1A, Ferris
-
Description
Plays a central role in allergic response
-
Recombinant
Yes
-
Source
HEK293 cells
-
Purity by SDS PAGE
≥90%
-
Assay
SDS-PAGE
-
Biological activity
Measured by its binding ability in a functional ELISA. Immobilized human FCER1A at 2 ug/ml (100 ul/well). The concentration of Human IgE that produces 50% of the optimal binding response is found to be approximately 0.1 - 0.4 µg/ml.
-
Results
Measured by its binding ability in a functional ELISA. Immobilized human FCER1A at 2 ug/ml (100 ul/well). The concentration of Human IgE that produces 50% of the optimal binding response is found to be approximately 0.1 - 0.4 µg/ml.
-
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 21.9 kDa. The predicted N-terminus is Val 26. DTT-reduced Protein migrates as 40-60 kDa due to glycosylation.
-
Storage Temp
-20°C
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Appearance
Lyophilized
-
Physical form description
Lyophilized from 0.22 um filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
-
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
-
Background Information
High affinity immunoglobulin epsilon receptor subunit alpha (FCER1A) is also known as Fc-epsilon RI-alpha (FcERI), IgE Fc receptor subunit alpha, FCE1A. FCER1A contains two Ig-like (immunoglobulin-like) domains. FCER1A binds to the Fc region of immunoglobulins epsilon and is a high affinity receptor. FCER1A is responsible for initiating the allergic response, which binding of allergen to receptor-bound IgE leads to cell activation and the release of mediators (such as histamine) responsible for the manifestations of allergy. The same receptor also induces the secretion of important lymphokines. FCER1A plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Gene symbol
FCER1A
-
Short name
CellExp™ FCER1A /FCE1A, recombinant
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Species
Humans
-
Alternative name
H. sapiens CellExp™ fragment c fragment on immunoglobuline E, high affinity I, receptor to measure; a polypeptide /FCE1A, H. sapiens Rec.
-
Alternative technique
rec
-
Alternative to gene target
Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide, FCE1A and FcERI, FCER1A and IDBG-103887 and ENSG00000179639 and 2205, IgE binding, Cell surfaces, Fcer1a and IDBG-205782 and ENSMUSG00000005339 and 14125, FCER1A and IDBG-630768 and ENSBTAG00000012887 and 506783
-
Gene info
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
