CD82, 50-53kD cell surface protein, Clone: B-L2, Mouse Monoclonal antibody-Human; frozen, IH/flow
-
Catalog numberOBT0601
-
PricePlease ask
-
Size200 tests
-
-
Alternative name 1CD82, 50-53kD cell surface protein, Clone: B-L2, Mab anti-Human; frozen, IH/flow
-
Alternative name 2CD82, 50-53kD cell surface protein, Clone: B-L2, Mab antibody-Human; frozen, IH/flow
-
Alternative name 3CD82, 50-53kD cell surface protein, Clone: B-L2, Mab antibody-Homo sapiens; frozen, IH/flow
-
Alternative name 4anti-CD82 50-53kD cell surface protein Clone: B-L2
-
Alternative name 5CD82 50-53kD cell surface protein Clone: B-L2
-
ClonalityMonoclonal Antibody
-
DescriptionThis is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against. If used correctly and according the protocol, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications. Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches; in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required.
-
Storage and handlingGenerally, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for longer periods - at -20 . Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°C.
-
AdvisoryTo keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided. For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container. Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom.
-
PropertiesIf you buy Antibodies supplied by accurate-monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional descriptionFor cells, cell lines and tissues in culture till half confluency.
-
TestFlow cytometry uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
-
Latin nameMus musculus
-
French translationanticorps
-
Gene target
-
Gene symbolGAP43, IGLV1-50, IGHV3-50, MRPL37, DCTN2, KLK5, SNRPB, ORM2, CD82, PPFIBP1
-
Short nameCD82, 50-53kD surface protein, Clone: B-L2, Mouse Monoclonal antibody- ; frozen, IH/
-
TechniqueMouse monoclonal, Antibody, frozen, Mouse, Flow, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, mouses, mouse monoclonals
-
Hostmouse monoclonal, Murine monoclonal antibodies or Mabs are often conjugated and the isotype is IgG.
-
SpeciesHuman, Humans, Mouses
-
Alternative nameCD82 molecule, 50-53kD cellular surface protein, clonality: B-L2, Mouse monoclonal (antibody to-)-H. sapiens; -25°C frozen, IH/flow
-
Alternative techniqueantibodies, murine
-
Alternative to gene targetCD82 molecule, 4F9 and C33 and GR15 and IA4 and KAI1 and R2 and SAR2 and ST6 and TSPAN27, CD82 and IDBG-40873 and ENSG00000085117 and 3732, protein binding, Plasma membranes, Cd82 and IDBG-192552 and ENSMUSG00000027215 and 12521, CD82 and IDBG-636903 and ENSBTAG00000031252 and 100335281,506713
-
Tissuecell, frozen
-
Gene info
-
Identity
-
Gene
-
Long gene namegrowth associated protein 43
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1990-07-10
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin lambda variable 1-50 (non-functional)
-
Synonyms gene name
- immunoglobulin lambda variable 1-50
-
GenBank acession
-
Locus
-
Discovery year2000-05-08
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin lambda locus at 22q11.2
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable 3-50 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable 3-50
- immunoglobulin heavy variable 3-50 pseudogene
-
GenBank acession
-
Locus
-
Discovery year2000-04-04
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemitochondrial ribosomal protein L37
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2001-02-28
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Large subunit mitochondrial ribosomal proteins
- Mitochondrial ribosomal proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namedynactin subunit 2
-
Synonyms gene name
- dynactin 2 (p50)
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1995-10-03
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Dynactin subunits
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namekallikrein related peptidase 5
-
Synonyms gene name
- kallikrein 5
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-03-16
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Kallikreins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namesmall nuclear ribonucleoprotein polypeptides B and B1
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year1988-11-28
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Small nucleolar RNA protein coding host genes
- Sm spliceosomal proteins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameorosomucoid 2
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year1991-03-11
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Lipocalins
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameCD82 molecule
-
Synonyms gene
-
Synonyms gene name
- kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))
- CD82 antigen
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1994-01-10
-
Entrez gene record
-
RefSeq identity
-
Classification
- Tetraspanins
- CD molecules
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namePPFIA binding protein 1
-
Synonyms gene name
- PTPRF interacting protein, binding protein 1 (liprin beta 1)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1998-10-23
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Sterile alpha motif domain containing
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
-
Tree numbers
- E01.370.225.500.363.342
- E01.370.225.500.386.350
- E05.196.712.516.600.240.350
- E05.200.500.363.342
- E05.200.500.386.350
- E05.242.363.342
- E05.242.386.350
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data