Oncostatin M, rat recombinant
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Catalog number
7182-50
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Price
Please ask
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Size
50 ug
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Synonyms
OSM
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Alternative_names
OSM
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Description
Participates in the regulation of neurogenesis, osteogenesis and hematopoiesis.
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Recombinant
Yes
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Source
E. Coli
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Purity by SDS PAGE
≥98%
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Assay
SDS-PAGE
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Endotoxin Level
< 0.1 ng/ug of protein (
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Activity Specifications test method
Assay #1: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 3.0-5.0 ug/ml. Assay #2: Determined by its ability to inhibit Alkaline phosphatase activity of differentiated MC3T3 E1 cells.
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Biological activity
Assay #1: Determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 is 3.0-5.0 ug/ml. Assay #2: Determined by its ability to inhibit Alkaline phosphatase activity of differentiated MC3T3 E1 cells.
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Molecular Weight
24.4 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized powder
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Physical form description
Sterile filtered through a 0.2 micron filter. Lyophilized with no additives
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Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
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Background Information
Oncostatin M (OSM) is a growth and differentiation factor that participates in the regulation of neurogenesis, osteogenesis and hematopoiesis. Produced by activated T cells, monocytes and Kaposi’s sarcoma cells, OSM can exert both stimulatory and inhibitory effects on cell proliferation. It stimulates the proliferation of fibroblasts, smooth muscle cells and Kaposi’s sarcoma cells, but, inhibits the growth of some normal and tumor cell lines. It also promotes cytokine release (e.g. IL-6, GM-CSF and G-CSF) from endothelial cells, and enhances the expression of low-density lipoprotein receptor in hepatoma cells. OSM share several structural and functional characteristics with LIF, IL-6, and CNTF. Human OSM is active on murine cells. Recombinant rat Oncostatin M is a 24.4 kDa protein, containing 215 amino acid residues.
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Amino acid sequence
MKRGCSSSSP KLLSQLKSQA NITGNTASLL EPYILHQNLN TLTLRAACTE HPVAFPSEDM LRQLSKPDFL STVHATLGRV WHQLGAFRQQ FPKIQDFPEL ERARQNIQGI RNNVYCMARL LHPPLEIPEP TQADSGTSRP TTTAPGIFQI KIDSCRFLWG YHRFMGSVGR VFEEWGDGSR RSRRHSPLWA WLKGDHRIRP SRSSQSAMLR SLVPR
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
OSMR, OSM, IL6STP1
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Short name
Oncostatin M, recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Host
Rat
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Species
Rat, Rats
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Alternative name
Oncostatin M, rat Rec.
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Alternative technique
rec
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Gene info
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Identity
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Gene
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Long gene name
oncostatin M receptor
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year
1999-02-17
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Fibronectin type III domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene name
oncostatin M
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-07-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Interleukin 6 type cytokine family
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VEGA ID
Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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