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Human Recombinant Ubiquitin K27

Human Recombinant Ubiquitin K27 is available 1 time from Biovision labs

6397-1000 | Human Recombinant Ubiquitin-K27 size: 1 mg | 251.88 USD

Catalog number 6397-1000
Supplier Biovision
Price 251.88 USD
Size 1 mg
Description Serves as a substrate for K27 polyubiquitination
Purity by SDS-PAGE ≥95%
Assay RP-HPLC
Background Information Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have all available lysines mutated to arginines, except at position 27. This molecule, therefore, can only form polyubiquitin chains of K27 linkage type. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.
Product images
    #Human#.jpg#0#.jpegHuman Recombinant Ubiquitin-K27
Synonyms Human Recombinant Ubiquitin-K27
Alternates names NA
Taglines Serves as a substrate for K27 polyubiquitination
Biomolecule/Target NA
NCBI Gene ID #. 6233
NCBI Gene Symbol RPS27A
Gene Source Human
Accession # P62979
Recombinant Yes
Source E. Coli
Purity N/A
Endotoxin Level N/A
Activity (Specifications/test method) N/A
Biological activity N/A
Results N/A
Molecular Weight 8.732 kDa
Storage Temp. -80°C
Shipping Dry ice
Shelf Life 1 year
Concentration 4 mg/ml
Appearance Liquid
Physical form description 4 mg/ml in PBS.
Reconstitution Instructions N/A
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Additional source Recombinants or rec. proteins
Group recombinants
Gene targetUbiquitin-K27
Short name Recombinant Ubiquitin-K27
Technique Recombinant
Species Human
Alternative name H. sapiens recombinant Ubiquitin-K27
Alternative technique rec
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Recombinant Ubiquitin-K27 | Technique alternative | 01012196
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