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Synonyms
CAM, CALM
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Alternative_names
CAM, CALM
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Description
Binds to and regulates a multitude of different protein targets
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Recombinant
No
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Source
Bovine Brain
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
2.5 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of N-benzoyl-DL-arginine-pNA per minute at 25°C in 200 mM Tris-HCl, pH 7.8, with 20 mM CaCl2.
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Results
2.5 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of N-benzoyl-DL-arginine-pNA per minute at 25°C in 200 mM Tris-HCl, pH 7.8, with 20 mM CaCl2.
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Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes one µmole of N-benzoyl-DL-arginine-pNA per minute at 25°C in 200 mM Tris-HCl, pH 7.8, with 20 mM CaCl2.
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Molecular Weight
16 kDa
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Storage Temp
-20°C
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Shipping
Gel Pack
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Shelf Life
12 months
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Appearance
Lyophilized
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Physical form description
Lyophilized in 30 mM Hepes, pH 7.4, 1 mM CaCl2 and 0.1 mM DTT.
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Reconstitution Instructions
In water or aqueous buffer
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Background Information
Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM can also make use of the calcium stores in the endoplasmic reticulum, and the sarcoplasmic reticulum. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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