Recombinant Human CDKN2D / p19ink4d Protein (GST tag) 50µg
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Catalog number
PKSH030816-50µg
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Price
Please ask
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Size
50µg
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Synonym
INK4D;p19;p19-INK4D
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Activity
Immobilized human GST-CDKN2D at 10 μg/ml (100 μl/well) can bind biotinylated human GST-CDK4, The EC50 of biotinylated human GST-CDK4 is 0.52-1.2 μg/ml.
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Sequence
Met 10Leu 166
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Fusion tag
N-GST
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Accession
P55273
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Expressed Host
E. coli
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Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
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Purity
＞90 % as determined by SDS-PAGE
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Endotoxin
Please contact us for more information.
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Stability and Storage
Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Mol Mass
44.9 kDa
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AP Mol Mass
46 kDa
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Formulation
Lyophilized from sterile PBS, pH 7.5
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Reconstitution
Please refer to it for detailed information.
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Background
Cyclin-dependent kinase inhibitor 2D(also known as CDKN2D or p19ink4d), a member of the INK4 family of cyclin-dependent kinase (CDK) inhibitors, negatively regulates the cyclin D-CDK4/6 complexes, which promote G1/S transition by phosphorylating the retinoblastoma tumor-suppressor gene product. It is clearly shown that DNA repair is the main target of p19ink4d effect and that diminished apoptosis is a downstream event. Experiments has uncovered a role of p19INK4d as a regulator of DNA-damage-induced apoptosis and suggest that it protects cells from undergoing apoptosis by allowing a more efficient DNA repair. It has been demonstrated that p19INK4d expression enhances cell survival under genotoxic conditions. Previous work has shown that inactivation of the cyclin-dependent kinase inhibitor (CKI) p19(Ink4d) leads to progressive hearing loss attributable to inappropriate DNA replication and subsequent apoptosis of hair cells. It may also involved in male reproductive function including testicular atrophy, alteration in serum follicle stimulating hormone, qualitative increase in germ cell apoptosis, and delayed kinetics of meiotic prophase markers.
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Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
CDKN2D, MGST3
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Short name
Recombinant CDKN2D / p19ink4d Protein (GST tag) 50µg
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Elabscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
tag
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Species
Human, Humans
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Alternative name
Rec. H. sapiens cyclin-dependent phosphorylation catalyst inhibitor 2D (p19, inhibits cyclin-dependent kinase 4) / p19ink4d Protein (GST detection labelled) 50µg
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Alternative technique
rec
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Alternative to gene target
cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4), INK4D and p19 and p19-INK4D, CDKN2D and IDBG-27803 and ENSG00000129355 and 1032, protein kinase binding, nuclei, Cdkn2d and IDBG-738755 and ENSMUSG00000096472 and 12581, CDKN2D and IDBG-643316 and ENSBTAG00000010731 and 510244
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Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
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Tree numbers
- E05.196.401.250
- E05.301.300.230
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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