Annexin V-FITC Apoptosis Kit

  • Catalog number
    K101-400
  • Price
    Please ask
  • Size
    400 assays
  • Description
    Detects Apoptosis & Necrosis within 10 min.
  • Summary
    • Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy • Species reactivity- Mammalian • Kit size- Convenient sizes (25, 100, 400 assays) • Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  • Sample Type
    Living cells (suspension and adherent)
  • Features Benefits
    • Simple one step staining procedure in 10 minutes • Fast and convenient • Kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining
  • Storage Conditions
    +4°C
  • Shipping Conditions
    gel pack
  • Shelf life
    12 months
  • Background
    Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining.
  • Additional description
    This Annexin V calcium dependent phospholipid binding protein will be downstream of BCL2 pathway and at caspase apoptotic level. Biovision has more cell death kits and antibodies. This 1 is suited for programmed cell-death studies.
  • Properties
    This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • Gene target
    Annexin   Kit  
  • Short name
    Annexin V-FITC Apoptosis Kit
  • Technique
    apoptosis, FITC, apoptosis pathway, Fluorescein
  • Label
    FITC
  • Alternative name
    Annexin V-fluorecein programmed cellular destruction reagent
  • Alternative technique
    celldeath, fluorescine, kits
  • Alternative to gene target
    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
MeSH Data
  • Name
  • Concept
    Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
  • Tree numbers
    • E01.370.225.500.386
    • E05.196.712.516.600.240
    • E05.200.500.386
    • E05.242.386
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products
Filters
Contact
Chat with gentaur.com employee