p53 Antibody (pSer315): HRP
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Catalog numberSPC-1411D-HRP
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PricePlease ask
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Size100 µl
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Targetp53 (pSer315)
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ConjugateHRP
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Research Area sCancer, Cell Cycle, p53 Pathway, Cancer, Tumor suppressors, Apoptosis, Cell Signaling, Epigenetics and Nuclear Signaling, DNA/RNA, DNA Damage and Repair
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Alternative Name sp53 tumor suppressor Antibody, Cellular tumor antigen p53 Antibody, Antigen NY-CO-13 Antibody, Phosphoprotein p53 Antibody, Tumor protein 53 Antibody, BCC7 Antibody, p53 Antibody, TP53 Antibody, P53_HUMAN Antibody, FLJ92943 Antibody, Mutant tumor protein 53 Antibody, Tumor protein p53 Antibody, LFS1 Antibody, Transformation related protein 53 Antibody, TRP53 Antibody, Tumor suppressor p53 Antibody
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CategoryAntibodies
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Product TypePolyclonal
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ImmunogenSynthetic phospho-peptide from human p53 around the phosphorylation site of serine 315 (pSer315).
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Immunogen SpeciesHuman
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Accession NumberNP_000537.3
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Gene ID7157
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Swiss ProtP04637
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ApplicationsWB, IHC, IP, ELISA
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Host SpeciesRabbit
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Species ReactivityHuman
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Antibody DilutionWB (1:250-1:1000); IHC (1:50-1:150); IP (1-2.5 ug per mg lysate); ELISA (1:5000); optimal dilutions for assays should be determined by the user.
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PurificationPeptide Affinity Purified
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Storage BufferPBS, 50% glycerol, 0.5% BSA, 0.02% sodium azide
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SpecificityDetects endogenous levels of p53 protein only when phosphorylated at serine 315 (pSer315). Detects a band at ~43 kDa.
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Storage Temperature-20ºC
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Shipping TemperatureBlue Ice or 4ºC
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Cellular LocalizationMitochondrion Matrix, Endoplasmic Reticulum, Nucleus Pml Body, Nucleus, Pml Body, Cytoplasm, Cytosol, Mitochondrion, Nuclear Chromatin, Nuclear Matrix, Nucleolus, Nucleoplasm, Intracellular, Protein Complex, Replication Fork
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Field of UseFor in vitro research use only.
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Gross Weight kg1.4
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Net Weight g0.1
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PropertiesIf you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
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ConjugationHRP Conjugated
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French translationanticorps
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Gene target
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Short namep53 Antibody (pSer315):
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelHRP
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Alternative namep53 (antibody to-) (pSer315): horseradish peroxidase
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data