MDM2 (S160) polyclonal antibody

• Catalog number
  BS1447-50ul
• Price
  Please ask
• Size
  50ul

• Alternative Name
  E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2; MDM2
• Immunogen
  Synthetic peptide, corresponding to amino acids 130-180 of Human MDM2.
• Swiss Prot
  Q00987
• Reactivity
  Human,Mouse,Rat
• Applications
  WB IHC IF
• Application_all
  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
• Product
  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Purification Purity
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
• Storage Stability
  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Specificity
  MDM2 (S160) polyclonal antibody detects endogenous levels of MDM2 protein.
• Usage
  Western blot (WB) analysis of MDM2 (S160) polyclonal antibody at 1:500 dillution Lane1:HeLa whole cell lysate(30μg) Lane2:PC12 whole cell lysate(30μg) Lane3:sp2/0 whole cell lysate(30μg)
• BiowMW
  ~ 55,90 kDa
• Note
  For research use only, not for use in diagnostic procedure.
• Properties
  If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Group
  Polyclonals and antibodies
• About
  Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
• French translation
  anticorps

• Gene target
  MDM2   S160  
• Gene symbol
  MDM2
• Short name
  MDM2 (S160) polyclonal antibody
• Technique
  Polyclonal, Antibody, antibodies against human proteins, antibodies for, Polyclonal antibodies (pAbs) are mostly rabbit or goat antibodies that are secreted by different B cells, whereas monoclonal antibodies come from a single N cell lineage. Pabs are a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope.
• Host
  Rabbit
• Alternative name
  MDM2 (S160) polyclonal (antibody to-)
• Alternative technique
  polyclonals, antibodies

Gene info

• Identity
  HGNC:6973
• Gene
  MDM2
• Long gene name
  MDM2 proto-oncogene
• Synonyms gene name
  • mouse double minute 2, human homolog of; p53-binding protein
  • Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
  • Mdm2 p53 binding protein homolog (mouse)
  • MDM2 proto-oncogene, E3 ubiquitin protein ligase
• Synonyms
  • HDM2
  • MGC5370
• Locus
  12q15
• Discovery year
  1993-12-10
• Entrez gene record
  4193
• Pubmed identfication
  • 1614537
  • 16905769
  • 29123033
• RefSeq identity
  • NM_002392
• Classification
  • Zinc fingers RANBP2-type
  • Ring finger proteins
• VEGA ID
  OTTHUMG00000142827

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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