Target of this antibody:
Alternative names of antibody target:
SET1, SET1A, KMT2F
SETD1A is a component of the SET1 histone methyltransferase (HMT) complex. This complex specifically methylates lysine 4 of histone H3, but only if the neighboring lysine 9 residue is not yet methylated. Methylation of H3K4 represents a specific tag for epigenetic transcriptional activation. SETD1A shows a non-overlapping localization with SETD1B, suggesting that both proteins play a specific role in the epigenetic control of chromatin structure and gene expression.
Clonality of antibody:
This antibody reacts with:
Polyclonal antibody raised in rabbit against mouse Setd1a (Set domain containing 1A), using three KLH-conjugated synthetic peptides: two containing an amino acid sequence from the N-terminal part of the protein and one containing an amino acid sequence from the central part of the protein
Concentration of antibody:
Form of antibody:
Formulation of this antibody:
In PBS with 0.05% (W/V) sodium azide.
This antibody has been succesfully tested for use in following methods:
Western blot, ELISA.
ELISA: 1:100, Western Blot: 1:500.
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Polyclonals and antibodies
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
anti-Setd1a polyclonal antibody
Polyclonal, Antibody, anti
antibody to-Setd1a polyclonal (Antibody to)