Alternative names of antibody target:
MYC , C-MYC , c-Myc
Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
This antibody reacts with:
Synthetic peptide corresponding to residues surrounding amino acid 413 of human c-Myc
Concentration of antibody:
Formulation of this antibody:
100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Myc-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal.
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation, Immunofluorescence
Western blotting (1-2 µg/ml), immunoprecipitation (20-40 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect endogenous levels of the transcription factor c-Myc.
Long gene name MYC binding protein
Discovery year 1999-11-26
Entrez gene record 26292
Havana BLAST/BLAT OTTHUMG00000000484