Human Recombinant LDHA

• Catalog number
  6374-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Human Recombinant LDHA
• Alternative_names
  L-lactate dehydrogenase A chain, GSD11, LDH1, LDHM, PIG19.
• Description
  Catalyzes the final step of anaerobic glycolysis
• Recombinant
  Yes
• Source
  E. Coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Biological activity
  Specific activity is >20 units/mg.
• Results
  >20 units/mg
• Unit Definition
  One unit will convert 1.0 µmole of pyruvate to L-lactate and β-NAD per minute at pH 7.5 at 37°C.
• Molecular Weight
  38.8 kDa (352 aa, 1-332 aa + His-Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  0.5 mg/ml
• Appearance
  Liquid
• Physical form description
  0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl and 20% glycerol.
• Background Information
  LDHA catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. LDHA is localized primarily in muscle tissue and is part of the lactate dehydrogenase family. Mutations in LDHA have been linked to exertional myoglobinuria. LDH1 is decreased in essential thrombocythemia. LDHA is induced through a non-genomic pathway of estrogen action. Reduction in LDH-A activity results in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  LDHA  
• Gene symbol
  LDHA
• Short name
  Recombinant LDHA
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  H. sapiens Rec. LDHA
• Alternative technique
  rec

Gene info

• Identity
  HGNC:6535
• Gene
  LDHA
• Long gene name
  lactate dehydrogenase A
• GenBank acession
  • X02152
• Locus
  11p15.1
• Discovery year
  2001-06-22
• Entrez gene record
  3939
• Pubmed identfication
  • 3000353
• RefSeq identity
  • NM_005566
• VEGA ID
  OTTHUMG00000167721

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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