14-3-3-Gamma (Western Blot Control) Protein

Storage

For short term storage 14-3-3-Gamma (Western Blot Control) Protein may be kep at +4 deg. C. For long term storage keep the aliquoted vials frozen at -20 degrees Celsius.
Tips

Due to handling and transportation, small amounts of the 14-3-3-Gamma (Western Blot Control) Protein may get caught on the walls or seal of the vials. Prior to use briefly rund the vial(s) through a centrifuge to collect all of the content on the bottom. Avoid freezing and thawing the vials numerous times as such cycles may denaturate the peptide chains and influence the item's performance.
Test

Western Blot can be performed on tissues and cell extracts to determine the proteins present in your sample after PAGE electrophoresis and transfer on PVDF or Nitrocellulose. PVDF is more advised. The best detection method is using enhanced chemiluminescence or ECL.
Description

Isotype or positive controls by peptides, antibodies and deactivated samples.

Identity

HGNC:12854
Gene

YWHAQ
Long gene name

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta
Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
- protein, theta
Synonyms
- HS1
- 14-3-3
Synonyms name
- protein tau
- 14-3-3 theta
GenBank acession
- AF070556
Locus

2p25.1
Discovery year

1999-08-20
Entrez gene record

10971
Pubmed identfication
- 11080204
RefSeq identity
- NM_006826
Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
VEGA ID

OTTHUMG00000013848

Identity

HGNC:12852
Gene

YWHAG
Long gene name

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma
Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide
Synonyms
Synonyms name
- 14-3-3 gamma
- protein phosphatase 1, regulatory subunit 170
GenBank acession
- AF142498
Locus

7q11.23
Discovery year

1993-09-20
Entrez gene record

7532
Pubmed identfication
- 10486217
- 10433554
RefSeq identity
- NM_012479
Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
VEGA ID

OTTHUMG00000022862

Identity

HGNC:12855
Gene

YWHAZ
Long gene name

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
Synonyms gene
- YWHAD
Synonyms gene name
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide
- tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Synonyms
- KCIP-1
- 14-3-3-zeta
Synonyms name
- 14-3-3 zeta
- 14-3-3 delta
GenBank acession
- U28964
Locus

8q22.3
Discovery year

1993-09-20
Entrez gene record

7534
Pubmed identfication
- 8617504
- 7890696
RefSeq identity
- NM_145690
Classification
- 14-3-3 phospho-serine/phospho-threonine binding proteins
VEGA ID

OTTHUMG00000134291

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data