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Protein Agarose antibody Antibody Purification Reagent

Protein Agarose antibody Antibody Purification Reagent is available 5 times from Nordc labs

X1195 | Protein G Agarose antibody Antibody Purification Reagent size: 1mL | 152.48 USD

Catalog number X1195
Supplier nordc
Price 152.48 USD
Size 1mL
Long description This outstanding recombinant Protein G has the albumin binding domain deleted providing very clean immunoglobin purifications. It is coupled to highly cross-linked agarose at 2 mg rProtein G per ml beads using cyanogen bromide activation. The combination provides very superior performance binding 20 mg of human IgG per ml beads under static conditions. Binding under flow will be less depending on the flow rate for all Protein A and Protein G products. High flow rates are possible with this highly cross-linked agarose product under gravity flow, but it is not designed to pump under high pressure.
Other description Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Antigen-antibody binding interaction Protein G Agarose antibody Antibody Purification Reagent
Antibody's reacts with see techfile
Application Antibody Purification
Antibody's suited for Ligand Density: approximately 2 mg Protein G per ml of drained gel _x000B_Dynamic Binding Capacity: approximately 18 mg human IgG per ml of drained gel (calculated as the amount of human IgG adsorbed to the gel before flow exceeded 1% of the absorbance of the ingoing solution at 30 cm/hour and a 0.92 mg/ml sample concentration) _x000B_Bead Structure: 4% highly cross-linked agarose _x000B_Bead Size Range: 45 to 185 µm _x000B_Mean Bead Size: approximately 90 µm _x000B_Maximum Linear Flow Rate: >1300 cm/hour at 0.05 MPa (0.5 bar) in an XK 16/20 column, bed height 5 cm _x000B_Maximum Operating Backpressure: 0.1 MPa (1 bar, 14 psi) _x000B_pH Working Range: 2 to 9 (NOTE: Protein G may hydrolyse at low pH) _x000B_pH Stability: Long term storage and cleaning-in-place - 3 to 9 _x000B_Chemical Stability: IgG binding capacity and recovery maintained after storage for (a) 7 days at 37¼ C in 1M acetic acid (pH 2.0), 2 mM sodium phosphate, 1% SDS (pH 7.0), 6 M guanidine-HCl, 70% ethanol or (b) 2 hours at room temperature in 0.1 M HCl, pH 1.0, 8 M urea, pH 10.5, 0.1 M Glycine-NaOH, pH 11 _x000B_Physical stability: Negligible volume variation due to changes in pH or ionic strength _x000B_Sanitization: Sanitize column using 70% ethanol
Properties The purest agarose was used in the production of Protein G Agarose antibody Antibody Purification Reagent by nordc.If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Conjugation Agarose
Test Antibodies are affinity purified with an antigen coated column or protein A or G agarose or beads. DNA is purified with endotoxin free silica columns or anion exchange resins. nordc supplies purification kits and ultra pure reagents.
French translation anticorps
Gene targetProtein Antibody Reagent
Short name Protein G Agarose antibody Antibody Purification Reagent
Technique Purification, Antibody, Agarose
Alternative name Protein G molecular sieve (Antibody to) Antibody purification Reagent

X1196 | Protein G Agarose antibody Antibody Purification Reagent size: 5mL | 303.78 USD

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X1198 | Protein G Agarose antibody Antibody Purification Reagent size: 25mL | 907.82 USD

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X1197 | Protein G Agarose antibody Antibody Purification Reagent size: 10mL | 499.66 USD

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X1204 | Protein G Agarose IP-Ready antibody Antibody Purification Reagent size: 1,5mL | 140.75 USD

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Category Other Reagents
Antibody come from n/a
Clone not specified
Antibody is raised in see techfile
Antibody's reacts with these species This antibody doesn't cross react with other species
Antibody's specificity No Data Available
Storage 4ºC
Relevant references no information yet
Protein number see ncbi
Warnings This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Isotype not specified
Label unlabeled
Alternative technique purifications
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