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Synonyms
PRL-R, Prolactin receptor, Lactogen receptor, Prlr, PRL, luteotropic hormone, LTH, PRLR, PRL-R, PRL R
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Alternative_names
PRL-R, Prolactin receptor, Lactogen receptor, Prlr, PRL, luteotropic hormone, LTH, PRLR, PRL-R, PRL R
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Description
A cytokine receptor
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥97%
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Assay
SDS-PAGE
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Purity
≥97%
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Biological activity
Determined by the dose-dependent inhibition of Prolactin stimulated proliferation of Nb2 cells and by high affinity binding of ovine Prolactin and other lactogenic hormones.
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Molecular Weight
24.12 kDa
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Storage Temp
-20°C
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Shipping
Blue ice
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Sterile filtered and lyophilized from a concentrated (1 mg/ml) solution with 0.0045 mM NaHCO₃
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Reconstitution Instructions
Reconstitute in sterile dH₂O to a concentration of 0.1 -1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers
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Background Information
Prolactin is a pituitary hormone that plays a role in the stimulation of milk production, salt and H₂O regulation, growth, development and reproduction. The primary step in its action is the binding to a specific membrane receptor (prolactin receptor) which belongs to the superfamily of class 1 cytokine receptors. Prolactin is a hormone involved in a range of significant functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. Prolactin exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane Prolactin receptor. PRLR varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. Recombinant human Prolactin Receptor (Extra Cellular Domain) produced in E.Coli is a non-glycosylated, Polypeptide chain containing 206 amino acids and having a molecular mass of 24.12 kDa.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin name
Rattus norvegicus
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Additional description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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Additional source
Recombinants or rec. proteins
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Group
recombinants
