HSP70, salmonid heat shock protein 70, Affinity purified

• Catalog number
  AS05 061A
• Price
  Please ask
• Size
  200 µg

• Available ordering format
  Lyophilized in PBS pH 7.4
• Immunogen
  KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified serum
• How to reconstitute
  For reconstitution add 200 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), immunoprecipiation (IP), immunohistochemistry (IHC)
• Connected products
  collection of antibodies to fish related targetsSecondary antibodies
• Recommended dilutions for use
  1:15 000 with standard ECL (WB), 5 µg of antibodies in reaction mixture (IP), 1: 100 (IHC)
• Molecular weight expected аpparent
  70 kDa
• Verified reactivity
  rainbow trout (Oncorhynchus mykiss)
• Possible reactivity
  atlantic salmon (Salmo salar), brook trout (Salvelinus fontinalis)
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  This antibody is recognizing the inducible Hsp70 in salmon but not the constitutive.
• References
  Curie et al. (2008). β-Adrenergic Stimulation Enhances the Heat-Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.
• Scientific context
  Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.
• Notes
  immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)
• Protein number
  B5X4Z3
• TAIR number
  Refer to NCBI
• Hyperlink
• Test
  A high affinity purification column was use to purify HSP70, salmonid heat shock protein 70, by agisera by chromatographic size exclusion.
• Description
  1 of protein A or G purified by agisera will give more specificity than crude serum.

• Gene target
  HSP70   salmonid   heat   shock   protein  
• Gene symbol
  HSPA1B, HSPA14, HSPA1A, HSPA1L
• Short name
  HSP70, salmonid heat shock protein 70,
• Technique
  affinity, purified, purifications
• Alternative name
  HSP70, salmonid heat shock protein 70, protein enriched
• Alternative technique
  pure

Gene info

• Identity
  HGNC:5233
• Gene
  HSPA1B
• Long gene name
  heat shock protein family A (Hsp70) member 1B
• Synonyms gene name
  • heat shock 70kD protein 1B
  • heat shock 70kDa protein 1B
• Synonyms
  • HSP70-2
• Locus
  6p21.33
• Discovery year
  1995-08-11
• Entrez gene record
  3304
• Pubmed identfication
  • 1700760
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031202

Gene info

• Identity
  HGNC:29526
• Gene
  HSPA14
• Long gene name
  heat shock protein family A (Hsp70) member 14
• Synonyms gene name
  • heat shock 70kDa protein 14
• Synonyms
  • HSP70-4
  • HSP70L1
• GenBank acession
  • AF112210
• Locus
  10p13
• Discovery year
  2004-04-20
• Entrez gene record
  51182
• Pubmed identfication
  • 21208456
• RefSeq identity
  • NM_016299
• Classification
  • Heat shock 70kDa proteins

Gene info

• Identity
  HGNC:5232
• Gene
  HSPA1A
• Long gene name
  heat shock protein family A (Hsp70) member 1A
• Synonyms gene
  • HSPA1
• Synonyms gene name
  • heat shock 70kD protein 1A
  • heat shock 70kDa protein 1A
• Synonyms
  • HSP70-1
• GenBank acession
  • BC002453
• Locus
  6p21.33
• Discovery year
  2001-06-22
• Entrez gene record
  3303
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031201

Gene info

• Identity
  HGNC:5234
• Gene
  HSPA1L
• Long gene name
  heat shock protein family A (Hsp70) member 1 like
• Synonyms gene name
  • heat shock 70kD protein-like 1
  • heat shock 70kDa protein 1-like
• Synonyms
  • HSP70-HOM
  • hum70t
• GenBank acession
  • D85730
• Locus
  6p21.33
• Discovery year
  1989-05-12
• Entrez gene record
  3305
• Pubmed identfication
  • 9685725
  • 9349405
• Classification
  • TIM23 complex
  • Heat shock 70kDa proteins
• VEGA ID
  OTTHUMG00000031208

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