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12 Hydroxyeicosatetraenoic Acid 12 HETE ELISA Kit 96 well plate

12 Hydroxyeicosatetraenoic Acid 12 HETE ELISA Kit 96 well plate is available 3 times from Bioma labs

EKU08719 | 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit, 96 well plate size: 10x96T | 8,486.83 USD

1. Gene info
Identity37557
GenePIRC53
Long gene name piwi-interacting RNA cluster 53
Locus12
Discovery year 2009-11-05
Entrez gene record100313861
Pubmed identfication
Classification
  • Piwi-interacting RNA clusters
MeSH Data
Concept
Scope note:An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
Additional information

EKU08719 | 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit, 96 well plate size: 5x96T | 4,693.07 USD

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EKU08719 | 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit, 96 well plate size: 96T | 1,110.70 USD

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Assay class Competitive
ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Samples to be analyzed Serum, plasma and other biological fluids
Estimated production time 7-11 business days
Sensitivity limit 4,64 ng/mL
Alternate gene name 12HETE
Gene number Please refer to GenBank
Assay duration 2 hours
QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Gene name 12-Hydroxyeicosatetraenoic Acid
Protein number Please refer to Uniprot
Detection limits 12.35-1000 ng/mL
Protocol Please see ELISA's datasheet, otherwise contact us
Use for 8 months
Notes For research use only. Not for diagnostic procedures.
Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Research main area Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology;
Shipping requirements Blue ice
ELISA detection Colorimetric
Verified reactivity Multispecies (General)
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of 12-Hydroxyeicosatetraenoic Acid (12-HETE). No significant cross-reactivity or interference between 12-Hydroxyeicosatetraenoic Acid (12-HETE) and analogues was observed.
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 12-Hydroxyeicosatetraenoic Acid (12-HETE) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 12-Hydroxyeicosatetraenoic Acid (12-HETE) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
Alternate protein number Please refer to SwissProt
Assay principle This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) and unlabeled 12-Hydroxyeicosatetraenoic Acid (12-HETE) (Standards or samples) with the pre-coated antibody specific to 12-Hydroxyeicosatetraenoic Acid (12-HETE). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 12-Hydroxyeicosatetraenoic Acid (12-HETE) in the sample.
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Verified applications ELISA
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Short name 12-Hydroxyeicosatetraenoic Acid (12-HETE) ELISA Kit, 96
Technique ELISA kit, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Alternative name 12-Hydroxyeicosatetraenoic Acid (12-HETE) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique elisas
Alternative to gene target v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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