C5a (His-Tag), murine recombinant
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Catalog number
4895A-20
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Price
Please ask
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Size
20 ug
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Synonyms
Complement Fragments C5a
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Alternative_names
Complement Fragments C5a, C5a anaphylatoxin chemotactic receptor
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Description
An anaphylatoxin
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Recombinant
Yes
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Source
E. coli
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Purity by SDS PAGE
≥95%
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Assay
SDS-PAGE
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Biological activity
ED₅₀ 1-3 nM as determined by enzyme release assay of myeloperoxidase
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Results
1-3 nmolar
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Molecular Weight
9 kDa
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Storage Temp
-20°C
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Shipping
Blue ice
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Shelf Life
12 months
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Appearance
Lyophilized protein
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Physical form description
Sterile filtered and lyophilized with no additives
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Reconstitution Instructions
Reconstitute to a concentration of 1.0 mg/ml in ice-cold H₂O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C.
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Background Information
Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. The 9 kDa mouse C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.
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Handling
Centrifuge the vial prior to opening.
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Usage
For Research Use Only! Not to be used in humans
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Conjugation
histidine
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Short name
C5a (His-Tag), murine recombinant
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture. Tags
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Label
His
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Alternative name
C5a (histidine-detection labelled), murine Rec.
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Alternative technique
rec
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MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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