PC-3 Cell Line
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Catalog numberEP-CL-0185
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PricePlease ask
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Size1×10⁶ cells/vial
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AbbreviationPC-3
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Leadtime3-5 days
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Shipping conditionDry ice
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StorageLiquid nitrogen -196°C
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Age62 years adult
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MorphologyEpithelial
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Growth_propertiesAdherent
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Doubling_timeNA
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BackgroundThe cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.
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Biosafety1
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MediumHam's F-12K (PM150910)+10% FBS (164210-500)+1% P/S (PB180120)
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SubculturingRemove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor. Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.
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Ratio1:2-1:4
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RenewalEvery 2 to 3 days
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CryopreservationFreeze medium: 60% Basal medium+30% FBS+10% DMSO Storage temperature: Liquid nitrogen vapor phase
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Culture_conditionsAtmosphere: Air, 95%; CO2, 5% Temperature: 37℃
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TumorigenicYes
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EffectsYes, in semi-solid medium; Yes, Tumors developed within 21 days at 100% frequency(5/5) in nude mice inoculated subcutaneously with 10^7 cells.
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Receptor_expressionNA
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Antigen_expressionHLA A1, A9
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Gene_expressionHla A1, A9
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ApplicationsTransfection host.
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DurationNA
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DescriptionFor cells, cell lines and tissues in culture till half confluency.
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Gene target
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Gene symbolMSMP, PC, CBX8, PKD1, BTG2, PCSK1, PODXL, ENPP1, PKD2, CDHR2
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Short namePC-3 Line
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SpeciesHomo sapiens, Human
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Alternative namePC-3 cellular Line
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TissueProstate, derived from metastatic site: bone
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Gene info
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Identity
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Gene
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Long gene namemicroseminoprotein, prostate associated
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Synonyms
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GenBank acession
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Locus
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Discovery year2009-02-27
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepyruvate carboxylase
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Synonyms
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GenBank acession
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Locus
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Discovery year1991-09-13
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- MicroRNA protein coding host genes
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namechromobox 8
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Synonyms gene name
- chromobox homolog 8 (Drosophila Pc class)
- chromobox homolog 8
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Chromobox family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepolycystin 1, transient receptor potential channel interacting
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Synonyms gene name
- polycystic kidney disease 1 (autosomal dominant)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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Classification
- MicroRNA protein coding host genes
- C-type lectin domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameBTG anti-proliferation factor 2
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Synonyms gene name
- BTG family, member 2
- BTG family member 2
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Synonyms
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Synonyms name
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Locus
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Discovery year2000-02-29
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- BTG/Tob family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameproprotein convertase subtilisin/kexin type 1
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Synonyms gene
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Synonyms gene name
- neuroendocrine convertase 1
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-06-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Proprotein convertase subtilisin/kexin family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepodocalyxin like
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Synonyms gene name
- podocalyxin-like
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Synonyms
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Locus
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Discovery year1997-07-11
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameectonucleotide pyrophosphatase/phosphodiesterase 1
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year1992-12-08
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Entrez gene record
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Pubmed identfication
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Classification
- Ectonucleotide pyrophosphatase/phosphodiesterase family
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namepolycystin 2, transient receptor potential cation channel
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Synonyms gene name
- polycystic kidney disease 2 (autosomal dominant)
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-08-07
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Transient receptor potential cation channels
- EF-hand domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namecadherin related family member 2
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Synonyms gene
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Synonyms gene name
- protocadherin 24
- cadherin-related family member 2
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2008-01-18
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Cadherin related
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VEGA ID
