Immunogen Purified IgG from dog serum
Purification Protein G purified IgG
Storage condition Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C to avoid repeated freeze and thaw cycles.
Verified applications ELISA (ELISA), Western Blot (WB)
Connected products Other anti-dog secondary antibodies
Recommended dilutions for use needs to be determined by end user
Verified reactivity dog IgG
No reactivity no confirmed exceptions from predicted reactivity known in the moment
References to be added when available antibody released in October 2015.
Scientific context Rabbit anti-dog IgG, Biotin conjugated is a secondary antibody which binds to all dog IgGs in immunological assays.
Notes Antibody is supplied in 0.01M PBS, PH 7.4.
Protein number Refer to NCBI
Properties Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
About Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Latin name Oryctolagus cuniculus