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Human Beta Site APP Cleaving Enzyme 1 bACE1 ELISA Kit 96 well plate

Human Beta Site APP Cleaving Enzyme 1 bACE1 ELISA Kit 96 well plate is available 3 times from Bioma labs

EKU02709 | Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit, 96 well plate size: 96T | 925.62 USD

Catalog number EKU02709
Supplier bioma
Price 925.62 USD
Size 96T
1. Gene info
Identity 55075
Gene APP-DT
Long gene name APP divergent transcript
Locus 21q21.3
Discovery year 2020-06-12
Classification
  • Divergent transcripts
Product images
    #Human#.jpg

EKU02709 | Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit, 96 well plate size: 10x96T | 7,064.74 USD

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EKU02709 | Human Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit, 96 well plate size: 5x96T | 3,909.23 USD

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Verified reactivity Homo sapiens (Human)
Detection limits 0.250-16 ng/mL
Sensitivity limit 0,11 ng/mL
Samples to be analyzed Serum, plasma, cerebrospinal fluid and other biological fluids
Assay duration 3 hours
Verified applications ELISA
Assay class Sandwich
ELISA detection Colorimetric
Protein number P56817
Alternate protein number Please refer to SwissProt
Gene number 23621
Gene name Beta-Site APP Cleaving Enzyme 1
Alternate gene name ASP2; Beta Secretase; Memapsin 2; Aspartyl protease 2; Beta-site amyloid precursor protein cleaving enzyme 1; Membrane-associated aspartic protease 2
Estimated production time 7-11 business days
Shipping requirements Blue ice
Storage recommendation -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Use for 8 months
ELISA's specificity This assay has high sensitivity and excellent specificity for detection of Beta-Site APP Cleaving Enzyme 1 (bACE1). No significant cross-reactivity or interference between Beta-Site APP Cleaving Enzyme 1 (bACE1) and analogues was observed.
ELISA's cross-reactivity This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
Assay principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-Site APP Cleaving Enzyme 1 (bACE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-Site APP Cleaving Enzyme 1 (bACE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-Site APP Cleaving Enzyme 1 (bACE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-Site APP Cleaving Enzyme 1 (bACE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Protocol Please see ELISA's datasheet, otherwise contact us
Precision of the test Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Site APP Cleaving Enzyme 1 (bACE1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Site APP Cleaving Enzyme 1 (bACE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
ELISA's stability The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
QC The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Research main area Neuro science;
Warnings The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Notes For research use only. Not for diagnostic procedures.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Description Enzymes are cleaving the substrate. If the substrate is DNA they are called restriction enzymes. Activating enzymes will cut off the domain that is biological active to become functional.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
Gene targetBeta-Site APP Cleaving bACE1
Short name Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit, 96
Technique ELISA kit, Enzyme, ELISA
Host antigen assay ELISA kit 1 Kit (96 Wells)
Species Human
Alternative name H. sapiens b-Site amyloid beta (A4) precursor protein Cleaving Enzyme 1 (bACE1) Enzyme-linked immunosorbent assay reagent, 96 well plate
Alternative technique enzymes
Alternative to gene target amyloid beta (A4) precursor protein, AAA and ABETA and ABPP and AD1 and APPI and CTFgamma and CVAP and PN-II and PN2, APP and IDBG-1100 and ENSG00000142192 and 351, growth factor receptor binding, nuclei, App and IDBG-172954 and ENSMUSG00000022892 and 11820, APP and IDBG-630293 and ENSBTAG00000017753 and 280722, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit, 96 | Tech
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  • Beta-Site
  • APP
  • Cleaving
  • bACE1
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